使用激光内窥镜和亚甲蓝进行根除治疗。
Eradication Using Laser Endoscope and Methylene Blue.
作者信息
Ogasawara Kouji, Nakajima Susumu, Sato Hiroshi, Sasaki Tadashi
机构信息
Sarufutsu Village National Health Insurance Hospital, Japan.
Asahikawa Medical University, Respiratory Center, Japan.
出版信息
Laser Ther. 2020 Jul 17;29(1):19-27. doi: 10.5978/islsm.20-OR-02.
BACKGROUND AND AIMS
() eradication has become increasingly unsuccessful due to the prevalence of antibiotic resistance. To address this global issue, a novel strategy for eradication without antibiotics must be developed. The purpose of this study was to examine the effect of methylene blue (MB) with sodium bicarbonate (NaHCO) on using photodynamic antimicrobial chemotherapy.
MATERIALS AND METHODS
MB was basified using NaHCO. The basic effect of MB with NaHCO was examined using an endoscope equipped with a laser light source. was smeared on the culture media with basic MB, followed by illumination at approximately 1,100 lux for 10 and 20 seconds.After 4 days of culture, the basic effects were determined according to the bacterial growth.
RESULTS
The basic effects of MB appeared at a pH from 8.6 to 9.0 and at NaHCO concentrations between 2% and 6.5%. MB concentrations of > 0.05% exhibited the basic effects. The duration of irradiation had no remarkable effects.
CONCLUSIONS
Our results showed that the laser endoscope and basic MB were effective for eradication.
背景与目的
由于抗生素耐药性的普遍存在，（某种细菌）根除变得越来越不成功。为解决这一全球性问题，必须开发一种无需抗生素的新型根除策略。本研究的目的是研究亚甲蓝（MB）与碳酸氢钠（NaHCO₃）联合使用光动力抗菌化疗对（某种细菌）的作用。
材料与方法
用NaHCO₃将MB碱化。使用配备激光光源的内窥镜检查MB与NaHCO₃的基本效果。将（某种细菌）涂抹在含有碱性MB的培养基上，然后在约1100勒克斯的光照下照射10秒和20秒。培养4天后，根据细菌生长情况确定基本效果。
结果
MB的基本效果出现在pH值为8.6至9.0以及NaHCO₃浓度在2%至6.5%之间时。MB浓度>0.05%时表现出基本效果。照射时间没有显著影响。
结论
我们的结果表明，激光内窥镜和碱性MB对（某种细菌）根除有效。
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