亚甲蓝光动力抗菌化疗（PACT）可增加白色念珠菌的膜通透性。
Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in Candida albicans.
作者信息
Giroldo Ligia Maria, Felipe Monalisa Poliana, de Oliveira Marco Antonio, Munin Egberto, Alves Leandro Procópio, Costa Maricilia Silva
机构信息
Instituto de Pesquisa e Desenvolvimento (IP&D), Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, SP, Brazil.
出版信息
Lasers Med Sci. 2009 Jan;24(1):109-12. doi: 10.1007/s10103-007-0530-2. Epub 2007 Dec 22.
Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines light and a photosensitizing drug, promoting a phototoxic effect on the treated cells, in general via oxidative damage. In this work we studied the effect of PACT, using methylene blue (MB), on the permeability of Candida albicans membrane. Our results demonstrated that the combination of MB and laser (684 nm) promoted a decrease in Candida growth. The inhibition was more pronounced in the presence of 0.05 mg/ml MB and with an energy density of 28 J/cm(2). The decrease in Candida growth was associated with an increase in membrane permeabilization. Thus, we suggest that a PACT mechanism using MB can be related to damage in the plasma membranes of the cells.
光动力抗菌化疗（PACT）是一种潜在的抗菌疗法，它将光和一种光敏药物结合起来，通常通过氧化损伤对被治疗细胞产生光毒性作用。在这项工作中，我们研究了使用亚甲蓝（MB）的光动力抗菌化疗对白色念珠菌细胞膜通透性的影响。我们的结果表明，MB与激光（684纳米）的联合使用促进了白色念珠菌生长的减少。在存在0.05毫克/毫升MB且能量密度为28焦/平方厘米的情况下，抑制作用更为明显。白色念珠菌生长的减少与膜通透性的增加有关。因此，我们认为使用MB的光动力抗菌化疗机制可能与细胞的质膜损伤有关。
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