Zhang Ning, Zhao Na, Xie Lu-Shuang, Huang Biao, Lin Si-Rui, Zhang Qun, Zhu Yuan-Bing, Wu Qiao-Feng, Yu Shu-Guang
Acupuncture and Moxibustion School, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China.
Institute of Acupuncture and Homeostasis Regulation, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, China.
Evid Based Complement Alternat Med. 2020 Aug 21;2020:2343817. doi: 10.1155/2020/2343817. eCollection 2020.
To study how thermal energy is converted after moxibustion at local skin from the view of mitochondrial respiratory chain and its key regulatory elements of sirtuins 1 (SIRT1) and sirtuins 3 (SIRT3).
Two moxibustion temperatures usually used in clinical practice (38°C and 46°C) were applied to Zusanli (ST36) acupoint for 30 minutes in C57BL/6J mice. Local skin samples were harvested at 30 min and 72 h after moxibustion intervention, respectively. The activity of mitochondrial respiratory chain complexes I-V was detected by spectrophotometry. The expression of SIRT1 and SIRT3 protein was detected by immunofluorescence staining or western blot.
Moxibustion at 38°C triggered more significant increase of mitochondrial respiratory chain complexes I-V expression. However, the protein expression of SIRT1 and SIRT3 at 46°C showed more obvious enhancement. In addition, the effect of mitochondrial respiratory chain complexes I-V activity on local skin of ST36 acupoint was more obvious at 30 min after moxibustion, while the expression of SIRT1 and SIRT3 protein was more significant at 72 h after moxibustion.
Mitochondrial respiratory chain and its key regulatory element proteins SIRT1 and SIRT3 play important role in the initial process of thermal energy conversion stimulated by different moxibustion temperatures in local skin.
从线粒体呼吸链及其关键调控元件沉默信息调节因子1（SIRT1）和沉默信息调节因子3（SIRT3）的角度，研究艾灸后局部皮肤的热能转化情况。
将临床常用的两种艾灸温度（38°C和46°C）施用于C57BL/6J小鼠的足三里（ST36）穴位30分钟。分别在艾灸干预后30分钟和72小时采集局部皮肤样本。采用分光光度法检测线粒体呼吸链复合体I-V的活性。通过免疫荧光染色或蛋白质印迹法检测SIRT1和SIRT3蛋白的表达。
38°C艾灸引发线粒体呼吸链复合体I-V表达更显著增加。然而，46°C时SIRT1和SIRT3的蛋白表达增强更明显。此外，艾灸后30分钟线粒体呼吸链复合体I-V活性对ST36穴位局部皮肤的影响更明显，而艾灸后72小时SIRT1和SIRT3蛋白的表达更显著。
线粒体呼吸链及其关键调控元件蛋白SIRT1和SIRT3在不同艾灸温度刺激局部皮肤的热能转化初始过程中起重要作用。
