Red propolis hydroalcoholic extract inhibits the formation of biofilms on denture surface.

BACKGROUND

To evaluate the antifungal activity of the red propolis hydroalcoholic extract (RPHE) against biofilms.

MATERIAL AND METHODS

The minimum inhibitory and fungicidal concentrations (MIC and MFC) of the RPHE were determined by the microdilution technique. biofilms were formed on the surface of resin specimens preconditioned with artificial saliva (1h). The specimens (N=48) were equally divided according to the four solutions used for anti-biofilm evaluation (n=12 per group). After overnight incubation, biofilms were daily exposed (2x/day for 15 min) along 3 days with 3% RPHE, 0.12% chlorhexidine (CHX), 50,000 IU/mL nystatin (NYS) or saline (0.9% NaCl). Biofilms were analyzed regarding the number of viable microorganisms (CFU/mL), the metabolic activity (MTT assay) and the proportion of hyphae (optical microscopy).

RESULTS

The MIC and MFC of RPHE were respectively 0.29 mg/mL (0.03%) and 1.17 mg/mL (0.12%). There was no difference in the microorganisms' viability (CFU/mL) among groups treated with RPHE (4.92×103), CHX (3.33×102) or NYS (6.8×104), being all of them different from NaCl (3.93×107) (<0.05). The CHX (0.133) had the lowest metabolic activity (<0.05), followed by RPHE (0.292) and NYS (0.302) (>0.05). All experimental groups had a mean proportion of hyphae <10%, lower than NaCl (70%).

CONCLUSIONS

RPHE has antifungal activity against biofilms, suggesting its use for the biofilm control on denture surfaces. Propolis, Candida albicans, biofilm, dentures, antifungal agents.