Plant Ecology and Environment Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India.
Plant Ecology and Environment Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India.
J Biotechnol. 2020 Nov 10;323:254-263. doi: 10.1016/j.jbiotec.2020.09.003. Epub 2020 Sep 6.
The study aims at investigating the efficacy of individual as well as combined application of AM fungi (Glomus macrocarpum) and ZnO nanoparticles on the uptake of lead and its toxicity in wheat (Triticum aestivum L.). The plants were grown in pots with different treatments of AM Fungi, ZnO NP, and Pb. The individual applications of AM fungi (Glomus macrocarpum) and ZnO NPs increased the growth and biochemical attributes of wheat and decreased the Pb uptake under Pb stress. The combined application of AM fungi (Glomus macrocarpum) and ZnO nanoparticles synergistically enhanced the overall growth performance of the plant and significantly reduced the uptake of Pb in wheat grown in Pb spiked soils. The combined application was effective, with 30.66 % increase in plant height, 30.62 % increase in plant fresh weight, 54.26 % increase in plant dry weight, 45.45 % increase in total chlorophyll content, 19.59 % increase in proline content, 26.65 % higher activity of SOD, 15.12 % higher activity of catalase (CAT), 17.69 % increase in HO content, 17.69 % increase in lipid peroxidation content, 52.09 % and 58.19 % decrease in Pb concentration in root and shoot of wheat, respectively, grown in Pb spiked soil (100 mg kg soil). The results indicate that combined application of AM fungi and ZnO nanoparticles can be a promising technique for the utilization of Pb-contaminated soils.
本研究旨在探讨丛枝菌根真菌（Glomus macrocarpum）和氧化锌纳米粒子（ZnO NPs）单独及联合应用对小麦（Triticum aestivum L.）吸收铅及其毒性的影响。将植物种植在不同处理的 AM 真菌、ZnO NPs 和 Pb 的盆中。单独应用 AM 真菌（Glomus macrocarpum）和 ZnO NPs 可增加小麦的生长和生化特性，并在 Pb 胁迫下降低 Pb 的吸收。AM 真菌（Glomus macrocarpum）和 ZnO 纳米粒子的联合应用协同增强了植物的整体生长性能，并显著降低了 Pb 污染土壤中小麦对 Pb 的吸收。联合应用效果显著，与单独处理相比，植物株高增加了 30.66%，鲜重增加了 30.62%，干重增加了 54.26%，总叶绿素含量增加了 45.45%，脯氨酸含量增加了 19.59%，SOD 活性增加了 26.65%，CAT 活性增加了 15.12%，HO 含量增加了 17.69%，脂质过氧化含量增加了 17.69%，Pb 浓度在根和地上部分的降幅分别为 52.09%和 58.19%，根和地上部分的 Pb 浓度分别为 100mgkg-1 土壤。结果表明，联合应用 AM 真菌和 ZnO 纳米粒子可能是一种利用 Pb 污染土壤的有前景的技术。
