Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Department of Botany, Bacha Khan University, Charsadda 24420, Pakistan.
Molecules. 2023 Feb 23;28(5):2091. doi: 10.3390/molecules28052091.
Iron oxide nanoparticles (FeO-NPs) were synthesized using extract as strong reducing and capping agents. The synthesized iron oxide nanoparticles IONPs were characterized by UV-visible spectroscopy, Fourier transform infrared (FTIR), X-ray diffractive analysis (XRD), scanning electron microscope (SEM), and Energy dispersive X-ray spectroscopy (EDX). IONPs synthesis was confirmed by UV-visible spectroscopy by observing the peak at 471 nm. Furthermore, different in vitro biological assays, which showed important therapeutic potentials, were performed. Antimicrobial assay of biosynthesized IONPs was performed against four different Gram-positive and Gram-negative bacterial strains. was found to be the least suspected strain (MIC: 35 µg/mL), and was found to be the most suspected strain (MIC: 14 µg/mL). The maximum antifungal assay was observed for (MIC: 27 µg mL). The cytotoxic assay of IONPs was also studied using a brine shrimp cytotoxicity assay, and LD value was reported as 47 µg/mL. In toxicological evaluation, IONPs was found to be biologically compatible to human RBCs (IC: >200 µg/mL). The antioxidant assay, DPPH 2,2-diphenyl-1-picrylhydrazyly was recorded at 73% for IONPs. In conclusion, IONPs revealed great biological potential and can be further recommended for in vitro and in vivo therapeutic purposes.
采用 提取物作为强还原剂和封端剂合成了氧化铁纳米粒子 (FeO-NPs)。通过紫外-可见光谱、傅里叶变换红外 (FTIR)、X 射线衍射分析 (XRD)、扫描电子显微镜 (SEM) 和能谱 (EDX) 对合成的氧化铁纳米粒子 IONPs 进行了表征。通过观察 471nm 处的峰，通过紫外-可见光谱证实了 IONPs 的合成。此外，还进行了不同的体外生物测定，这些测定显示了重要的治疗潜力。对生物合成的 IONPs 进行了针对四种不同的革兰氏阳性和革兰氏阴性细菌菌株的抗菌测定。结果发现 是最少可疑的菌株 (MIC: 35µg/mL)，而 是最可疑的菌株 (MIC: 14µg/mL)。观察到最大的抗真菌测定是针对 (MIC: 27µg/mL)。还使用盐水虾细胞毒性测定研究了 IONPs 的细胞毒性，LD 值报告为 47µg/mL。在毒理学评价中，发现 IONPs 对人 RBC 具有生物相容性 (IC: >200µg/mL)。抗氧化测定，DPPH 2,2-二苯基-1-苦肼基自由基，IONPs 的记录值为 73%。总之，IONPs 显示出巨大的生物学潜力，可以进一步推荐用于体外和体内治疗目的。
