Department of Environmental Sciences and Engineering, Government College University, Faisalabad, Pakistan.
Department of Bioinformatics & Biotechnology, Government College University, Faisalabad, Pakistan.
Environ Pollut. 2019 Nov;254(Pt B):113109. doi: 10.1016/j.envpol.2019.113109. Epub 2019 Aug 28.
The production and soil accumulation of nanoparticles (NPs) from the industrial sector has increased concerns about their toxic effects in plants which needs the research to explore the ways of reducing NPs toxicity in pants. The gibberellic acid (GA) has been found to reduce abiotic stresses in plants. However, the effect of GA in reducing zinc oxide (ZnO) NPs-mediated toxicity in plants remains unclear. In this study, foliar application of GA was used to explore the possible role in reducing ZnO NPs toxicity in wheat (Triticum aestivum L.) plants. The plants were grown in pots spiked with ZnO NPs (0, 300, 600, 900, 1200 mg/kg) and GA (0, 100, 200 mg/L) was foliar sprayed at different times during the growth period under ambient environmental conditions. Our results demonstrated that GA inhibited the toxicity of ZnO NPs in wheat especially at higher levels of NPs. The GA application improved the plant biomass, photosynthesis, nutrients, and yield under ZnO NPs stress. The GA reduced the Zn accumulation, and reactive oxygen species generation in plants caused by toxicity of NPs. The protective effect of GA in decreasing ZnO NPs-induced oxidative stress was related to GA-mediated enhancement in antioxidant enzymes in plants. The role of GA in enhancing tolerance of wheat against ZnO NPs was further confirmed by the enhancement in nutrient contents in shoots and roots of wheat. Overall, our study provides the evidence that GA can reduce ZnO NPs-induced toxicity in wheat and probably in other crops which needs further in-depth investigation.
工业部门生产和土壤积累的纳米颗粒（NPs）引起了人们对其在植物中产生毒性效应的担忧，这需要研究探索减少植物中 NPs 毒性的方法。赤霉素（GA）已被发现可减轻植物的非生物胁迫。然而，GA 降低氧化锌（ZnO）NPs 介导的植物毒性的作用尚不清楚。在这项研究中，叶面喷施 GA 被用于探索其在减轻小麦（Triticum aestivum L.）植物中 ZnO NPs 毒性方面的可能作用。将植物种植在盆栽中，盆栽中添加了 ZnO NPs（0、300、600、900、1200mg/kg），并在环境条件下的生长期间，在不同时间叶面喷施 GA（0、100、200mg/L）。我们的结果表明，GA 抑制了 ZnO NPs 对小麦的毒性，尤其是在更高水平的 NPs 下。GA 的应用提高了植物生物量、光合作用、养分和产量，减轻了 ZnO NPs 胁迫下的植物生物量、光合作用、养分和产量。GA 减少了 NPs 毒性引起的植物中 Zn 积累和活性氧物种的产生。GA 降低 ZnO NPs 诱导的氧化应激的保护作用与 GA 介导的植物抗氧化酶增强有关。GA 通过增强小麦对 ZnO NPs 的耐受性，进一步证实了 GA 在减少 ZnO NPs 诱导的氧化应激方面的作用，这可以从 shoots 和 roots 中的营养物质含量的提高中得到证实。总的来说，我们的研究提供了证据表明，GA 可以减轻小麦中 ZnO NPs 诱导的毒性，可能在其他作物中也是如此，这需要进一步深入研究。
