Department of General Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 200000, PR China.
Department of Oral and Maxillofacial-Head & Neck Oncology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 200000, PR China.
Int J Biochem Cell Biol. 2020 Oct;127:105846. doi: 10.1016/j.biocel.2020.105846. Epub 2020 Sep 6.
Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with high morbidity and mortality rates. In spite of numerous advancements have been made in therapeutic methods, the prognosis of HNSCC patients remains poor. Therefore, investigation of crucial genes during HNSCC tumorigenesis which could be exploited as biomarkers and therapeutic targets is greatly needed. In this study, original data of four independent datasets was downloaded from the Gene Expression Omnibus database and analyzed through R language to screen out differentially expressed genes. Paired like homeodomain 1 and SAM and SH3 domain-containing 1 were selected to be further explored through multiple online databases. Quantitative real-time polymerase chain reaction analysis and immunohistochemistry assay were adopted to validate the downregulation of paired like homeodomain 1 and SAM and SH3 domain-containing 1 in HNSCC and statistical analysis indicated their close associations with patient prognosis. In vitro experiments demonstrated the inhibitory effect of paired like homeodomain 1 and SAM and SH3 domain-containing 1 on HNSCC progression. Overall, we identified the aberrant downregulation of paired like homeodomain 1 and SAM and SH3 domain-containing 1 in HNSCC and suggested the potential of utilizing them as therapeutic targets or efficient biomarkers for diagnosis and prognosis evaluation. Our findings may provide novel evidences for the development of new strategies for HNSCC treatment.
头颈部鳞状细胞癌（HNSCC）是一种侵袭性恶性肿瘤，发病率和死亡率均较高。尽管在治疗方法上取得了许多进展，但 HNSCC 患者的预后仍然较差。因此，迫切需要研究 HNSCC 肿瘤发生过程中的关键基因，这些基因可以作为生物标志物和治疗靶点。在这项研究中，从基因表达综合数据库下载了四个独立数据集的原始数据，并通过 R 语言进行分析，以筛选出差异表达的基因。选择配对同源域 1 和 SAM 和 SH3 域包含 1 进行进一步探索，通过多个在线数据库进行。采用定量实时聚合酶链反应分析和免疫组织化学检测来验证 HNSCC 中配对同源域 1 和 SAM 和 SH3 域包含 1 的下调，并进行统计学分析表明它们与患者预后密切相关。体外实验证明了配对同源域 1 和 SAM 和 SH3 域包含 1 对 HNSCC 进展的抑制作用。总的来说，我们确定了配对同源域 1 和 SAM 和 SH3 域包含 1 在 HNSCC 中的异常下调，并提出了利用它们作为治疗靶点或高效生物标志物用于诊断和预后评估的潜力。我们的研究结果可能为 HNSCC 治疗的新策略的发展提供新的依据。
