Department of General Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China.
Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, National Clinical Research Center of Stomatology, 200000, P.R. China.
Biosci Rep. 2019 Jul 29;39(7). doi: 10.1042/BSR20191612. Print 2019 Jul 31.
Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy with high morbidity and mortality rates and ranks as the sixth most common cancer all over the world. Despite numerous advancements in therapeutic methods, the prognosis of HNSCC patients still remains poor. Therefore, there is an urgent need to have a better understanding of the molecular mechanisms underlying HNSCC progression and to identify essential genes that could serve as effective biomarkers and potential treatment targets. In the present study, original data of three independent datasets were downloaded from the Gene Expression Omnibus database (GEO) and R language was applied to screen out the differentially expressed genes (DEGs). PYGM and TNNC2 were finally selected from the overlapping DEGs of three datasets for further analyses. Transcriptional and survival data related to PYGM and TNNC2 was detected through multiple online databases such as Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), cBioportal, and UALCAN. Quantitative real-time polymerase chain reaction (qPCR) analysis was adopted for the validation of PYGM and TNNC2 mRNA level in HNSCC tissues and cell lines. Survival curves were plotted to evaluate the association of these two genes with HNSCC prognosis. It was demonstrated that PYGM and TNNC2 were significantly down-regulated in HNSCC and the aberrant expression of PYGM and TNNC2 were correlated with HNSCC prognosis, implying the potential of exploiting them as therapeutic targets for HNSCC treatment or potential biomarkers for diagnosis and prognosis.
头颈部鳞状细胞癌（HNSCC）是一种侵袭性恶性肿瘤，发病率和死亡率高，在全球范围内排名第六。尽管治疗方法有了许多进展，但 HNSCC 患者的预后仍然不佳。因此，迫切需要更好地了解 HNSCC 进展的分子机制，并确定可作为有效生物标志物和潜在治疗靶点的关键基因。在本研究中，从基因表达综合数据库（GEO）下载了三个独立数据集的原始数据，并应用 R 语言筛选出差异表达基因（DEGs）。最终从三个数据集的重叠 DEGs 中选择了 PYGM 和 TNNC2 进行进一步分析。通过 Oncomine、基因表达谱交互式分析（GEPIA）、cBioportal 和 UALCAN 等多个在线数据库检测与 PYGM 和 TNNC2 相关的转录和生存数据。采用实时定量聚合酶链反应（qPCR）分析验证 HNSCC 组织和细胞系中 PYGM 和 TNNC2 mRNA 水平。绘制生存曲线以评估这两个基因与 HNSCC 预后的相关性。结果表明，PYGM 和 TNNC2 在 HNSCC 中明显下调，PYGM 和 TNNC2 的异常表达与 HNSCC 预后相关，表明其具有作为 HNSCC 治疗的治疗靶点或诊断和预后的潜在生物标志物的潜力。
