Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
Carbohydr Res. 2020 Nov;497:108125. doi: 10.1016/j.carres.2020.108125. Epub 2020 Aug 17.
Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: →4)-α-l-GulpNAc3OAcAN-(1 →3)-β-d-QuipNAc-(1→ .
假单胞菌 ANT_J38B 是从南极洲的一个岛屿中分离出来的革兰氏阴性菌。LPS 使用苯酚/氯仿/石油醚法提取。温和酸水解后通过凝胶过滤纯化得到 O-链。多糖通过化学分析和 NMR 光谱进行表征。O-链显示具有以下骨架的二糖重复单元：→4）-α-l-GulpNAc3OAcAN-（1→3）-β-d-QuipNAc-（1→。
