de Vry J, Donselaar I, van Ree J M
Rudolf Magnus Institute for Pharmacology, Medical Faculty, University of Utrecht, The Netherlands.
Life Sci. 1988;42(26):2709-15. doi: 10.1016/0024-3205(88)90247-0.
The effect of the vasopressin neuropeptide desglycinamide9, (Arg8) vasopressin (DGAVP) on the acquisition of intravenous cocaine self-administration was studied. Rats were tested under conditions of reduced body weight in a continuous reinforcement operant procedure, during five daily 3 h sessions. Under these conditions, the rate of self-administration obtained with 0.125 and 0.25, but not 0.063 mg.ml-1 cocaine, exceeded the rate obtained with saline. Daily pretreatment with DGAVP (5 micrograms/rat, s.c.) decreased self-administration of 0.125 and 0.25 mg.ml-1 cocaine to the level obtained with saline, but had no effect on self-administration of 0.063 mg.ml-1 cocaine and saline. Using a similar procedure, it was shown that daily intracerebroventricular pretreatment with vasopressin antiserum significantly increased self-administration of 0.125 mg.ml-1 cocaine, without affecting self-administration of 0.063 and 0.25 mg.ml-1 cocaine and saline. The results support previous findings obtained with vasopressin neuropeptides in drug self-administration studies and suggest that DGAVP decreases the acquisition of cocaine self-administration by attenuating the reinforcing effects of cocaine and that endogenous vasopressin may be involved in the acquisition of cocaine self-administration.
研究了血管加压素神经肽去甘氨酰胺9，（精氨酸8）血管加压素（DGAVP）对静脉注射可卡因自我给药习得的影响。在连续强化操作性程序中，在体重减轻的条件下对大鼠进行测试，每天进行5次，每次3小时。在这些条件下，0.125和0.25（而非0.063mg/ml）可卡因的自我给药速率超过了生理盐水的给药速率。每天皮下注射DGAVP（5微克/只大鼠）预处理可使0.125和0.25mg/ml可卡因的自我给药量降至生理盐水的给药水平，但对0.063mg/ml可卡因和生理盐水的自我给药量没有影响。采用类似程序表明，每天脑室注射血管加压素抗血清预处理可显著增加0.125mg/ml可卡因的自我给药量，而不影响0.063和0.25mg/ml可卡因以及生理盐水的自我给药量。这些结果支持了先前在药物自我给药研究中使用血管加压素神经肽获得的发现，并表明DGAVP通过减弱可卡因的强化作用降低了可卡因自我给药的习得，且内源性血管加压素可能参与了可卡因自我给药的习得。
