Thesleff I, Viinikka L, Saxén L, Lehtonen E, Perheentupa J
Department of Pedodontics and Orthodontics, University of Helsinki, Finland.
Life Sci. 1988;43(1):13-8. doi: 10.1016/0024-3205(88)90231-7.
To clarify the production of human epidermal growth factor (EGF) by different salivary glands, we measured its concentration by radioimmunoassay separately in whole saliva, in parotid gland (PG) saliva and in mixed submandibular (SMG) and sublingual gland (SLG) saliva. Also, we studied the presence of EGF in PG and SMG by immunohistochemistry. The mean (geometric) concentrations of EGF in PG saliva (2704 pg/ml, +/- SEM interval 2393-3056 pg/ml, n = 20) was higher (p less than 0.001) than in whole saliva (864 pg/ml, +/- 733-1019 pg/ml, n = 29), which in turn was higher (p less than 0.001) than in mixed SMG + SLG saliva (357 pg/ml, +/- 296-430 pg/ml, n = 16). No sex difference existed in any salivary gland EGF. Immunohistochemistry revealed EGF in the acinar cells of both PG and SMG, but only in PG there were prominent EGF deposits in luminal spaces. Our data suggest that EGF is produced by both PG and SMG, but that more of it is secreted from the PG. This result is new and challenges the general view that human salivary EGF is mainly from SMG. In mouse almost all salivary EGF comes from SMG and its amount is androgen dependent. Thus there are great differences in sources and regulation of salivary EGF between man and mouse.
为了阐明不同唾液腺产生人表皮生长因子（EGF）的情况，我们通过放射免疫分析法分别测定了全唾液、腮腺（PG）唾液以及颌下腺（SMG）和舌下腺（SLG）混合唾液中EGF的浓度。此外，我们还通过免疫组织化学方法研究了PG和SMG中EGF的存在情况。PG唾液中EGF的平均（几何）浓度（2704 pg/ml，±SEM区间2393 - 3056 pg/ml，n = 20）高于全唾液（864 pg/ml，±733 - 1019 pg/ml，n = 29）（p < 0.001），而全唾液又高于SMG + SLG混合唾液（357 pg/ml，±296 - 430 pg/ml，n = 16）（p < 0.001）。各唾液腺EGF均不存在性别差异。免疫组织化学显示PG和SMG的腺泡细胞中均有EGF，但仅在PG的管腔间隙中有明显的EGF沉积。我们的数据表明，PG和SMG均产生EGF，但PG分泌的更多。这一结果是新的，挑战了人唾液EGF主要来自SMG的普遍观点。在小鼠中，几乎所有唾液EGF都来自SMG，其含量受雄激素依赖。因此，人与小鼠唾液EGF的来源和调节存在很大差异。
