Changes of Inertial Focusing Position in a Triangular Channel Depending on Droplet Deformability and Size.

Studies on cell separation with inertial microfluidics are often carried out with solid particles initially. When this condition is applied for actual cell separations, the efficiency typically becomes lower because of the polydispersity and deformability of cells. Therefore, the understanding of deformability-induced lift force is essential to achieve highly efficient cell separation. We investigate the inertial focusing positions of viscous droplets in a triangular channel while varying Re, deformability, and droplet size. With increasing Re and decreasing droplet size, the top focusing position splits and shifts along the sidewalls. The threshold size of the focusing position splitting increases for droplets with larger deformability. The overall path of the focusing position shifts with increasing Re also has a strong dependency on deformability. Consequently, droplets of the same size can have different focusing positions depending on their deformability. The feasibility of deformability-based cell separation is shown by different focusing positions of MCF10a and MCF7 cells.