Bottema C K, Parks L W
Biochim Biophys Acta. 1978 Dec 22;531(3):301-7. doi: 10.1016/0005-2760(78)90212-6.
An in vitro assay for delta14-sterol reductase from yeast was developed, using ergosta-8,14-dien-3beta-ol as the substrate. The kinetics and localization of the enzyme were examined. The inhibition of the enzyme by the antimycotic agent, 15-azasterol, was verified.
开发了一种利用麦角甾-8,14-二烯-3β-醇作为底物的酵母δ14-甾醇还原酶体外测定法。研究了该酶的动力学和定位。验证了抗真菌剂15-氮杂甾醇对该酶的抑制作用。