Yang Chunhong, Wei Qingqing, Kang Li, Tang Hui, Zhu Guiyu, Cui Xinxing, Chen Yuxia, Jiang Yunliang
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
Department of Biology Science and Technology, Taishan University, Taian 271021, China.
J Poult Sci. 2016 Apr 25;53(2):111-117. doi: 10.2141/jpsa.0140113.
The chaperonin containing TCP-1 complex protein 1 subunit zeta (CCT6A) is the only cytosolic chaperonin in eukaryotes assisting in the folding of cytoplasmic proteins. Previous study revealed that the mRNA expression of chicken gene was remarkably elevated in the sexually mature ovaries. However, the mechanism underlying chicken expression changes remains largely unknown. In this study, haplotypes caused by two single nucleotide polymorphisms (SNPs) of chicken gene promoter (g.-2215 T>C and g.-1959 T>C) were identified and their associations with egg production traits as well as effects on gene expression were analyzed. Altogether four haplotypes including A (C-T), B (C-C), C (T-T) and D (T-C) were detected in all of the five chicken populations. Diplotypes AA, AD and DD were predominant in Xinyang brown hens, among which diplotype AD was associated with higher egg number at the age of 28 weeks old (E28) (<0.05). In addition, diplotype AD was also predominant in Xinyang brown and Hy-line brown chicken populations with high egg production; whereas in Wenchang and Shouguang chicken populations which are Chinese indigenous chicken breeds and relatively lower in egg production, diplotype AA was predominant. Compared with diplotypes AA and DD, the mRNA expression of in diplotype AD birds is the highest in F1, F5, and POF1 follicles of Hy-line brown hens (<0.05). These results suggest that the two SNPs in chicken promoter region are potential DNA marker for improving egg production trait.
含TCP-1复合蛋白1亚基ζ的伴侣蛋白（CCT6A）是真核生物中唯一协助细胞质蛋白折叠的胞质伴侣蛋白。先前的研究表明，鸡基因的mRNA表达在性成熟卵巢中显著升高。然而，鸡表达变化的潜在机制仍 largely未知。在本研究中，鉴定了鸡基因启动子的两个单核苷酸多态性（SNP）（g.-2215 T>C和g.-1959 T>C）引起的单倍型，并分析了它们与产蛋性状的关联以及对基因表达的影响。在所有五个鸡群体中总共检测到四种单倍型，包括A（C-T）、B（C-C）、C（T-T）和D（T-C）。双倍型AA、AD和DD在信阳褐壳蛋鸡中占主导地位，其中双倍型AD与28周龄时较高的产蛋数（E28）相关（<0.05）。此外，双倍型AD在产蛋量高的信阳褐壳蛋鸡和海兰褐壳蛋鸡群体中也占主导地位；而在文昌鸡和寿光鸡群体（中国地方鸡种，产蛋量相对较低）中，双倍型AA占主导地位。与双倍型AA和DD相比，双倍型AD鸡在海兰褐壳蛋鸡的F1、F5和POF1卵泡中的mRNA表达最高（<0.05）。这些结果表明，鸡启动子区域的两个SNP是改善产蛋性状的潜在DNA标记。
