Shandong Jihua Poultry Breeding Co. Ltd, Rizhao, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
Anim Biotechnol. 2023 Nov;34(4):1413-1421. doi: 10.1080/10495398.2022.2028162. Epub 2022 Feb 21.
Annexin A2 () is a member of the A subfamily of a multifunctional calcium dependent membrane phospholipid binding protein family. The mRNA expression of is consistent with ovary function and egg laying in chickens. In this study, six nucleotide polymorphisms in the key promoter region of chicken gene (-2861bp to -1394bp), i.e.,: g.-2337 indel (GT), g.-2255 C>T, g. -2248A>G, g.-2188 A>G, g.-2169 G>A, g.-2160 A>C, were identified. Their distributions in populations of Xinyang Brown, Recessive White Rock, Wenchang and Wenshang Barred chickens were analyzed. In the Recessive White Rock chicken population, , and were three major haplotypes. Association analysis indicated that the individuals with diplotype / laid more eggs at 32weeks, and the individual with diplotype / laid at the earlier age. Luciferase activity assay showed that mutation from to at -2255 increased trascriptional activity of chicken , which is consistent with its effect on egg laying traits.
膜联蛋白 A2（）是多功能钙依赖性膜磷蛋白家族 A 亚家族的成员。在鸡中，的 mRNA 表达与卵巢功能和产蛋一致。在这项研究中，鉴定了鸡基因关键启动子区域（-2861bp 到-1394bp）中的六个核苷酸多态性，即：g.-2337 插入/缺失（GT）、g.-2255C>T、g.-2248A>G、g.-2188A>G、g.-2169G>A、g.-2160A>C。分析了信阳棕鸡、隐性白洛克鸡、文昌鸡和汶上芦花鸡群体中的分布。在隐性白洛克鸡群体中，、和是三个主要单倍型。关联分析表明，二倍体/个体在 32 周时产蛋更多，二倍体/个体产蛋更早。荧光素酶活性测定表明，-2255 处从 到 的突变增加了鸡的转录活性，这与其对产蛋性状的影响一致。
