大肠杆菌中dam甲基化介导的oriC解旋。
The oriC unwinding by dam methylation in Escherichia coli.
作者信息
Yamaki H, Ohtsubo E, Nagai K, Maeda Y
机构信息
Institute of Applied Microbiology, University of Tokyo, Japan.
出版信息
Nucleic Acids Res. 1988 Jun 10;16(11):5067-73. doi: 10.1093/nar/16.11.5067.
Abstract
It has been shown that dam methylation is important in the regulation of initiation of DNA replication in E.coli. The question then arises as to whether dam methylation in the oriC region mediates any structural changes in DNA involved in the regulation of initiation of DNA replication. We demonstrate that the thermal melting temperature of the oriC region is lowered by adenine methylation at GATC sites. The regulation of initiation of DNA replication by dam methylation may be attributed to the ease of unwinding at GATC sites in oriC.
摘要
已经表明，dam甲基化在大肠杆菌DNA复制起始的调控中很重要。那么问题就来了，oriC区域的dam甲基化是否介导了参与DNA复制起始调控的DNA结构变化。我们证明，oriC区域的热解链温度会因GATC位点的腺嘌呤甲基化而降低。dam甲基化对DNA复制起始的调控可能归因于oriC中GATC位点解链的容易程度。
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本文引用的文献
1
Promoters in the E. coli replication origin.大肠杆菌复制起点中的启动子。
Nature. 1981 Nov 26;294(5839):376-8. doi: 10.1038/294376a0.
2
Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication.大肠杆菌K-12染色体的复制起点：携带自主复制信息的最小DNA片段的大小和结构
Mol Gen Genet. 1980 Apr;178(1):9-20. doi: 10.1007/BF00267207.
3
Stability of proteins. Proteins which do not present a single cooperative system.蛋白质的稳定性。不存在单一协同体系的蛋白质。
Adv Protein Chem. 1982;35:1-104.
4
In vitro replication of a dam methylated and non-methylated ori-C plasmid.一种经DNA腺嘌呤甲基化酶甲基化和未甲基化的ori-C质粒的体外复制
J Mol Biol. 1984 Jun 15;176(1):155-9. doi: 10.1016/0022-2836(84)90386-3.
5
Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.两种新型修饰甲基化酶的纯化与特性研究：来自嗜热栖热放线菌L的MClaI和来自嗜热水栖热菌YTI的MTaqI
Nucleic Acids Res. 1981 Dec 21;9(24):6795-804. doi: 10.1093/nar/9.24.6795.
6
Initiation site of deoxyribonucleotide polymerization at the replication origin of the Escherichia coli chromosome.大肠杆菌染色体复制起点处脱氧核糖核苷酸聚合的起始位点。
Mol Gen Genet. 1983;189(3):422-31. doi: 10.1007/BF00325904.
7
Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript.由RNA I与引物转录本相互作用决定的质粒ColE1不相容性。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6096-100. doi: 10.1073/pnas.78.10.6096.
8
Conformation of N6-methyladenine, a base involved in DNA modification: restriction processes.
Science. 1973 Nov 23;182(4114):833-4. doi: 10.1126/science.182.4114.833.
9
Effects of methylation on the stability of nucleic acid conformations: studies at the monomer level.甲基化对核酸构象稳定性的影响：单体水平的研究
Biochemistry. 1974 Sep 24;13(20):4143-58. doi: 10.1021/bi00717a013.
10
Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12.大肠杆菌K12 DNA中6-甲基腺嘌呤残基的生物学功能。
J Mol Biol. 1974 May 15;85(2):309-22. doi: 10.1016/0022-2836(74)90366-0.
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