Yamaki H, Ohtsubo E, Nagai K, Maeda Y
Institute of Applied Microbiology, University of Tokyo, Japan.
Nucleic Acids Res. 1988 Jun 10;16(11):5067-73. doi: 10.1093/nar/16.11.5067.
It has been shown that dam methylation is important in the regulation of initiation of DNA replication in E.coli. The question then arises as to whether dam methylation in the oriC region mediates any structural changes in DNA involved in the regulation of initiation of DNA replication. We demonstrate that the thermal melting temperature of the oriC region is lowered by adenine methylation at GATC sites. The regulation of initiation of DNA replication by dam methylation may be attributed to the ease of unwinding at GATC sites in oriC.
已经表明，dam甲基化在大肠杆菌DNA复制起始的调控中很重要。那么问题就来了，oriC区域的dam甲基化是否介导了参与DNA复制起始调控的DNA结构变化。我们证明，oriC区域的热解链温度会因GATC位点的腺嘌呤甲基化而降低。dam甲基化对DNA复制起始的调控可能归因于oriC中GATC位点解链的容易程度。
