一种经DNA腺嘌呤甲基化酶甲基化和未甲基化的ori-C质粒的体外复制
In vitro replication of a dam methylated and non-methylated ori-C plasmid.
作者信息
Hughes P, Squali-Houssaini F, Forterre P, Kohiyama M
出版信息
J Mol Biol. 1984 Jun 15;176(1):155-9. doi: 10.1016/0022-2836(84)90386-3.
PMID:6330369
Abstract
We have examined the replication of a dam methylated and non-methylated ori-C plasmid in an in vitro ori-C dependent replication system. The results show that the non-methylated plasmid is 50% to 80% less efficient in the initiation of DNA synthesis; that the methylation state of the plasmid does not change the site of initiation at ori-C, and that in both cases initiation at this region requires the presence of exogenously furnished dnaA protein.
摘要
我们已经在一个体外ori-C依赖性复制系统中研究了一种经dam甲基化和未甲基化的ori-C质粒的复制情况。结果表明，未甲基化的质粒在DNA合成起始方面的效率要低50%至80%；质粒的甲基化状态不会改变ori-C处的起始位点，并且在这两种情况下，该区域的起始都需要外源提供的dnaA蛋白的存在。
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