胃炎患者七种重要（.）毒力因子与耐药性的相关性分析
Analysis of Correlation between the Seven Important (. ) Virulence Factors and Drug Resistance in Patients with Gastritis.
作者信息
Oktem-Okullu Sinem, Cekic-Kipritci Zehra, Kilic Elif, Seymen Nogayhan, Mansur-Ozen Nesteren, Sezerman Ugur, Gurol Yesim
机构信息
Department of Medical Microbiology, Acibadem Mehmet Ali Aydinlar University, School of Medicine, Atasehir, Istanbul, Turkey.
Yeditepe University Hospital, Istanbul, Turkey.
出版信息
Gastroenterol Res Pract. 2020 Aug 29;2020:3956838. doi: 10.1155/2020/3956838. eCollection 2020.
The aim of this study is to evaluate the association between seven important . virulence factors and antibiotic resistance in patients with gastritis. . strains isolated from 33 patients with gastritis were examined. Antimicrobial susceptibilities were tested by GenoType® HelicoDR (Hain Life Science, Germany) test kit and RT-PCR. The virulence-factors were determined using conventional PCR. 39% of patients were resistant for clarithromycin and 27% of patients were resistant for fluoroquinolone. 15% of patients were resistant to both clarithromycin and fluoroquinolone. The . genotype was the most frequent allelic combination. Patients were possessed the , (6.1%); , (6.1%); , (15.1%); and , (3.0%) genotypes. 94% of patients with gastritis were positive for . gene. Also, there were no gene-positive gastritis patients. There was no significant correlation between the , , , , , , , , virulence genes, clarithromycin, and fluoroquinolone resistance. Herein, we report that the relationship between the . gene and gastritis. Although we found a correlation between . virulence factor and clinical outcome, there is a need for further studies to enlighten the relation between . virulence genes and antibiotic resistance.
本研究旨在评估胃炎患者中七种重要毒力因子与抗生素耐药性之间的关联。对从33例胃炎患者中分离出的菌株进行了检测。采用GenoType® HelicoDR（德国海因生命科学公司）检测试剂盒和逆转录聚合酶链反应（RT-PCR）检测抗菌药物敏感性。使用常规聚合酶链反应（PCR）确定毒力因子。39%的患者对克拉霉素耐药，27%的患者对氟喹诺酮耐药。15%的患者对克拉霉素和氟喹诺酮均耐药。该基因型是最常见的等位基因组合。患者拥有（6.1%）；（6.1%）；（15.1%）；和（3.0%）基因型。94%的胃炎患者基因呈阳性。此外，没有基因阳性的胃炎患者。、、、、、、、、毒力基因、克拉霉素和氟喹诺酮耐药性之间无显著相关性。在此，我们报告基因与胃炎之间的关系。尽管我们发现毒力因子与临床结果之间存在相关性，但仍需要进一步研究以阐明毒力基因与抗生素耐药性之间的关系。
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