1Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Antimicrob Resist Infect Control. 2018 Sep 29;7:115. doi: 10.1186/s13756-018-0409-y. eCollection 2018.
Foods with animal origins and particularly milk play a considerable role in transmission of . The current study was performed to assess phenotypic characters of antibiotic resistance and genotyping pattern of , , , and alleles amongst the strains isolated from raw milk.
Six-hundred and thirty raw milk samples were collected and cultured on Wilkins Chalgren anaerobe media. Antibiotic resistance and genotyping patterns were studied using disk diffusion and PCR, respectively.
Sixty-seven out of 630 (10.63%) raw milk samples were positive for . Ovine raw milk (17.27%) samples had the highest prevalence of , while camel (5.00%) had the lowest. strains harbored the highest prevalence of resistance against ampicillin (82.08%), tetracycline (76.11%), amoxicillin (74.62%), metronidazole (65.67%) and erythromycin (53.73%). Prevalence of resistance against more than 10 types of antibiotics was 17.91%. (83.58%), (80.59%), (77.61%) and (68.65%), (73.13%) and (44.77%) were the most commonly detected genotypes. We found that (56.71%), (56.71%), (43.28%) and (43.28%) were the most commonly detected genotyping pattern. Frequency of -, - and - genotypes were 26.86%, 62.68% and 55.22%, respectively. We found that S1a/cagA+/iceA1/oipA-/babA2- (28.35%), m1a/cagA+/iceA1/oipA-/babA2- (28.35%) and s2/cagA+/iceA1/oipA-/babA2- (26.86%) were the most commonly detected combined genotyping pattern.
Simultaneous presence of , , , and genotypes in antibiotic resistant strains indicates important public health issue regarding the consumption of raw milk. However, additional researches are required to find molecular genetic homology and other epidemiological aspects of in milk.
动物源性食品，尤其是牛奶，在传播 方面发挥了相当大的作用。本研究旨在评估从生奶中分离出的 菌株对抗生素的耐药表型特征和基因型模式，以及 、 、 和 等位基因。
采集 630 份生奶样本，接种于威尔金斯查尔格伦厌氧菌培养基上。采用纸片扩散法和 PCR 法分别检测抗生素耐药性和基因分型模式。
630 份生奶样本中，有 67 份（10.63%）为 阳性。绵羊生奶（17.27%）样本中 的检出率最高，而骆驼（5.00%）的检出率最低。 菌株对氨苄西林（82.08%）、四环素（76.11%）、阿莫西林（74.62%）、甲硝唑（65.67%）和红霉素（53.73%）的耐药率最高。对 10 种以上抗生素耐药的比例为 17.91%。 （83.58%）、 （80.59%）、 （77.61%）和 （68.65%）、 （73.13%）和 （44.77%）是最常见的基因型。我们发现， （56.71%）、 （56.71%）、 （43.28%）和 （43.28%）是最常见的基因分型模式。-、-和-基因型的频率分别为 26.86%、62.68%和 55.22%。我们发现，S1a/cagA+/iceA1/oipA-/babA2-（28.35%）、m1a/cagA+/iceA1/oipA-/babA2-（28.35%）和 s2/cagA+/iceA1/oipA-/babA2-（26.86%）是最常见的组合基因型模式。
耐药 菌株同时存在 、 、 、 和 基因型，这表明食用生奶存在重要的公共卫生问题。然而，需要进一步的研究来发现 基因在牛奶中的分子遗传同源性和其他流行病学方面。
