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肯尼亚霍马湾县传统加工木薯(克兰茨)产品中的真菌和黄曲霉毒素水平

Fungi and Aflatoxin Levels in Traditionally Processed Cassava ( Crantz) Products in Homa Bay County, Kenya.

作者信息

Obong'o Boniface Oure, Ayodo George, Kawaka Fanuel, Adalla Morelly Kathy

机构信息

School of Health Sciences, Jaramogi Oginga Odinga University of Science and Technology, P.O. Box 210, Bondo, Kenya.

Kenya Industrial Research and Development Institute, P.O. Box 6017, Kisumu, Kenya.

出版信息

Int J Microbiol. 2020 Aug 26;2020:3406461. doi: 10.1155/2020/3406461. eCollection 2020.

Abstract

Cassava ( Crantz) is a major source of carbohydrates, calcium, vitamins (B and C), and essential minerals and is the third most important source of calories in the tropics. However, it is not clear if the traditional processing methods expose the products to microbial contamination. This study assessed the levels of fungi and aflatoxin contamination in traditionally processed cassava products ( and ). A total of 38 samples were collected from the local markets in 7 subcounties in Homa Bay County, Kenya. The levels of aflatoxin were determined using an indirect competitive ELISA protocol. Yeast and mould contamination was determined using ISO 21527-2 method. Mean aflatoxin levels in chopped, fermented, and sun-dried cassava ( were 0.36 g/kg compared to 0.25 g/kg in chopped and sun-dried ( products. Aflatoxin contamination was detected in 55% of the samples and ranged from 0-5.33 g/kg. These levels are within 10 g/kg recommended by the CODEX STAN 193-1995. Yeast and mould counts in fermented and chopped sun-dried products were 3.16 log Cfu/g and 2.92 log Cfu/g, respectively. The yeast and mould counts were above standards set by East African Standard 739:2010 in 62% () and 58% (). The most prevalent fungal species were (68.4%) and (68%) followed by (18.4%), (15.8%) (15.8%), and spp. (5.3%). spp. was the only mycotoxigenic mould isolated from the samples. The study shows that cassava consumers are exposed to the risk of aflatoxin poisoning. The study, therefore, recommends appropriate surveillance to ensure safety standards.

摘要

木薯(Crantz)是碳水化合物、钙、维生素(B族和C族)以及必需矿物质的主要来源,是热带地区第三大热量来源。然而,传统加工方法是否会使产品受到微生物污染尚不清楚。本研究评估了传统加工木薯产品中的真菌和黄曲霉毒素污染水平。共从肯尼亚霍马湾县7个乡的当地市场采集了38个样本。使用间接竞争ELISA方法测定黄曲霉毒素水平。采用ISO 21527-2方法测定酵母和霉菌污染情况。切碎、发酵和晒干木薯中的平均黄曲霉毒素水平为0.36克/千克,而切碎和晒干(产品中的平均水平为0.25克/千克。55%的样本检测到黄曲霉毒素污染,范围为0 - 5.33克/千克。这些水平在CODEX STAN 193-1995推荐的10克/千克以内。发酵和切碎晒干产品中的酵母和霉菌计数分别为3.16 log CFU/克和2.92 log CFU/克。62%()和58%()的酵母和霉菌计数高于东非标准739:2010规定的标准。最常见的真菌种类是(68.4%)和(68%),其次是(18.4%)、(15.8%)(15.8%)和 spp.(5.3%)。 spp.是从样本中分离出的唯一产毒霉菌。该研究表明木薯消费者面临黄曲霉毒素中毒风险。因此,该研究建议进行适当监测以确保安全标准。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82cd/7471817/341eb3dfc0ca/ijmicro2020-3406461.001.jpg

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