Surono Ingrid S, Venema Koen
Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta 11480, Indonesia.
Centre for Healthy Eating & Food Innovation, Maastricht University-Campus Venlo, St. Jansweg 20, 5928 RC, Venlo, Netherlands.
Int J Microbiol. 2020 Aug 18;2020:8893283. doi: 10.1155/2020/8893283. eCollection 2020.
To investigate the effect of flour and starch of the Indonesian native tuber "taro" on the composition and activity of the gut microbiota in diabetic rats, streptozotocin (STZ)-induced diabetic rats were fed normal chow (AIN), or AIN in which corn starch was replaced by either taro flour or purified taro starch for 4 weeks. Fecal samples were collected at baseline and after 4 weeks, and the composition of microbial communities was measured using 16S rRNA sequencing, while SCFAs were measured using ion chromatography. Bodyweight declined upon DM induction with STZ. Feeding taro starch led to a lower reduction in bodyweight than feeding taro starch, but this was only significant for taro starch in weeks 2, 3, and 4 ( = 0.02, = 0.01, and < 0.01, respectively). Both taro starch and taro flour induced changes in the gut microbiota composition compared to AIN, which were different for taro flour and taro starch. , , and were markers for taro flour feeding, while was a marker for taro starch feeding. Induction of diabetes also led to changes in the microbiota composition. Random Forest correctly predicted for 16 of 18 samples whether rats were diabetic or not and correctly predicted 6 of 12 microbiota samples belonging to either taro flour- or taro starch-fed groups, indicating also some significant overlap in the substrate, as expected. Taro starch and taro flour both led to a significant increase in the fecal concentrations of acetate, propionate, and butyrate.
为研究印度尼西亚本土块茎“芋头”的面粉和淀粉对糖尿病大鼠肠道微生物群组成及活性的影响，将链脲佐菌素（STZ）诱导的糖尿病大鼠分为三组，分别喂食普通饲料（AIN）、用芋头粉或纯化芋头淀粉替代玉米淀粉的AIN饲料，持续4周。在基线期和4周后采集粪便样本，使用16S rRNA测序测定微生物群落组成，同时使用离子色谱法测定短链脂肪酸（SCFAs）。用STZ诱导糖尿病后大鼠体重下降。喂食芋头淀粉导致的体重降低幅度低于喂食芋头粉，但仅在第2、3和4周时，芋头淀粉组体重降低有显著差异（分别为P = 0.02、P = 0.01和P < 0.01）。与AIN组相比，芋头淀粉和芋头粉均诱导了肠道微生物群组成的变化，芋头粉和芋头淀粉的影响有所不同。[具体微生物名称1]、[具体微生物名称2]和[具体微生物名称3]是喂食芋头粉的标志物，而[具体微生物名称4]是喂食芋头淀粉的标志物。糖尿病的诱导也导致了微生物群组成的变化。随机森林分析正确预测了18个样本中的16个大鼠是否患有糖尿病，并且正确预测了属于芋头粉或芋头淀粉喂养组的12个微生物群样本中的6个，这也表明如预期的那样，在底物方面存在一些显著重叠。芋头淀粉和芋头粉均导致粪便中乙酸盐、丙酸盐和丁酸盐浓度显著增加。
