Department of Emergency Medicine, First People's Hospital of Lianyungang, Hospital of the Clinical Medical School of Nanjing Medical University, Lianyungang 222002, China.
Department of Intensive Care Unit, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China.
Comput Math Methods Med. 2020 Aug 25;2020:8741739. doi: 10.1155/2020/8741739. eCollection 2020.
Neonatal sepsis is one of the most prevalent causes of death of the neonates. However, the mechanisms underlying neonatal sepsis remained unclear. The present study identified a total of 1128 upregulated mRNAs and 1008 downregulated mRNAs, 28 upregulated lncRNAs, and 61 downregulated lncRNAs in neonatal sepsis. Then, we constructed PPI networks to identify key regulators in neonatal sepsis, including ITGAM, ITGAX, TLR4, ITGB2, SRC, ELANE, RPLP0, RPS28, RPL26, and RPL27. lncRNA coexpression analysis showed HS.294603, LOC391811, C12ORF47, LOC729021, HS.546375, HNRPA1L-2, LOC158345, and HS.495041 played important roles in the progression of neonatal sepsis. Bioinformatics analysis showed DEGs were involved in the regulation cellular extravasation, acute inflammatory response, macrophage activation of NF-kappa B signaling pathway, TNF signaling pathway, HIF-1 signaling pathway, Toll-like receptor signaling pathway, and ribosome, RNA transport, and spliceosome. lncRNAs were involved in regulating ribosome, T cell receptor signaling pathway, RNA degradation, insulin resistance, ribosome biogenesis in eukaryotes, and hematopoietic cell lineage. We thought this study provided useful information for identifying novel therapeutic markers for neonatal sepsis.
新生儿败血症是导致新生儿死亡的最常见原因之一。然而，新生儿败血症的发病机制尚不清楚。本研究在新生儿败血症中鉴定出了 1128 个上调的 mRNAs、1008 个下调的 mRNAs、28 个上调的 lncRNAs 和 61 个下调的 lncRNAs。然后，我们构建了 PPI 网络，以鉴定新生儿败血症中的关键调节剂，包括 ITGAM、ITGAX、TLR4、ITGB2、SRC、ELANE、RPLP0、RPS28、RPL26 和 RPL27。lncRNA 共表达分析显示 HS.294603、LOC391811、C12ORF47、LOC729021、HS.546375、HNRPA1L-2、LOC158345 和 HS.495041 在新生儿败血症的进展中发挥重要作用。生物信息学分析表明，DEGs 参与了细胞渗出、急性炎症反应、NF-κB 信号通路、TNF 信号通路、HIF-1 信号通路、Toll 样受体信号通路和核糖体、RNA 转运和剪接体的调节。lncRNAs 参与调节核糖体、T 细胞受体信号通路、RNA 降解、胰岛素抵抗、真核生物核糖体生物发生和造血细胞谱系。我们认为这项研究为鉴定新生儿败血症的新治疗标志物提供了有用的信息。
