Pokrovskaya L A, Nadezhdin S V, Zubareva E V, Burda Yu E, Gnezdyukova E S
National Research Tomsk State University, Tomsk, Russia.
Research Laboratory "Cellular, Assisted Reproductive, and DNA Technologies", Belgorod State National Research University, Belgorod, Russia.
Bull Exp Biol Med. 2020 Aug;169(4):571-575. doi: 10.1007/s10517-020-04931-5. Epub 2020 Sep 10.
We studied the expression of transcription factors RUNX2 and Osterix after addition of a concentrate of osteogenic-conditioned medium to the culture medium for osteogenic differentiation of mesenchymal stem cells (MSC). The obtained concentrate of osteogenic-conditioned medium containing a complex of bioactive substances with a molecular weight >10 kDa provided MSC differentiation into osteoblasts, which was confirmed by high level of expression of transcription factors RUNX2 and Osterix in comparison with the negative control. The highest expression of transcription factor Osterix was revealed on day 14 of MSC culturing in the presence of osteogenic supplement StemPro (positive control) and the studied concentrate of osteogenic-conditioned medium.
我们在向间充质干细胞（MSC）成骨分化培养基中添加成骨条件培养基浓缩物后，研究了转录因子RUNX2和Osterix的表达情况。所获得的成骨条件培养基浓缩物含有分子量>10 kDa的生物活性物质复合物，可促使MSC分化为成骨细胞，与阴性对照相比，转录因子RUNX2和Osterix的高表达证实了这一点。在存在成骨补充剂StemPro（阳性对照）和所研究的成骨条件培养基浓缩物的情况下，MSC培养第14天时转录因子Osterix的表达最高。
