Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand.
Bioresour Technol. 2020 Dec;318:124057. doi: 10.1016/j.biortech.2020.124057. Epub 2020 Aug 31.
Utilizing light energy for hydrogen production by combining nano-bio inspired photosynthetic system has received remarkable attention in renewable energy production. In this study, we applied a sodium alginate encapsulation strategy to our previously developed nano-bio hybrid system for photocatalytic hydrogen production under aerobic condition by combining the inorganic semiconductor titanium dioxide (TiO), electron mediator methylviologen (MV), along with E. coli (hydrogenases) in an encapsulated system under the given light intensity of 2000 W m and its hydrogen production efficiency was studied. Under aerobic condition the encapsulated hybrid system produced hydrogen (2.4 mmol H/ mmol glucose) 3-fold higher than the unencapsulated hybrid system (0.8 mmol H/ mmol glucose), suggesting that encapsulation is essential to protect oxygen sensitive hydrogenase under aerobic condition. The encapsulated hybrid system was also feasible under direct sunlight for hydrogen production. Overall, this study could serve as a new strategy for biological hydrogen production under aerobic condition.
利用纳米-生物启发的光合作用系统将光能转化为氢气的方法在可再生能源生产中受到了广泛关注。在本研究中，我们应用海藻酸钠包封策略，将先前开发的纳米-生物杂化系统应用于有氧条件下的光催化氢气生产中，该系统结合了无机半导体二氧化钛（TiO）、电子介体甲紫精（MV）以及大肠杆菌（氢化酶），在给定的 2000 W/m2 光强下进行包封系统，研究了其产氢效率。在有氧条件下，包封杂化系统产生的氢气（2.4 mmol H/mmol 葡萄糖）是未包封杂化系统（0.8 mmol H/mmol 葡萄糖）的 3 倍，这表明包封对于保护有氧条件下对氧气敏感的氢化酶是必要的。该包封杂化系统也可在直接阳光下进行氢气生产。总的来说，本研究可为有氧条件下的生物产氢提供一种新策略。
