Ramprakash Balasubramani, Incharoensakdi Aran
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand.
Bioresour Technol. 2023 Nov;388:129703. doi: 10.1016/j.biortech.2023.129703. Epub 2023 Aug 27.
The photocatalytic nano-biohybrid systems have great potential for the conversion of solar energy to fermentative hydrogen production. Herein, a whole-cell nano-biohybrid system consisting of biosynthesized cadmium sulfide, Enterobacter aerogenes cells, and metal oxide nanoparticles was constructed. The system was encapsulated with sodium alginate and used for light-driven biohydrogen production under anaerobic and in the presence of oxygen conditions. After 48 h incubation in the presence of oxygen, the E. aerogenes cells with the encapsulated hybrid system yielded 2.7 mmol H/mmol glucose, a 13.5-fold higher than that of the E. aerogenes cells without encapsulation. The encapsulated hybrid system could produce hydrogen for up to 96 h and could produce hydrogen even under natural sunlight conditions. These results revealed that efficient hydrogen production is possible in the presence of oxygen. Overall, the present study demonstrated the potential of using proper nano-biohybrid system with encapsulation for the production of hydrogen under ambient air condition.
光催化纳米生物杂交系统在将太阳能转化为发酵产氢方面具有巨大潜力。在此，构建了一种由生物合成的硫化镉、产气肠杆菌细胞和金属氧化物纳米颗粒组成的全细胞纳米生物杂交系统。该系统用海藻酸钠包封，并用于在厌氧和有氧条件下的光驱动生物制氢。在有氧条件下孵育48小时后，带有包封杂交系统的产气肠杆菌细胞产生了2.7 mmol H/mmol葡萄糖，比未包封的产气肠杆菌细胞高13.5倍。包封的杂交系统可以产氢长达96小时，甚至在自然阳光条件下也能产氢。这些结果表明在有氧存在的情况下高效产氢是可能的。总体而言，本研究证明了使用合适的包封纳米生物杂交系统在环境空气条件下生产氢气的潜力。
