Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2022 Jan;344(Pt B):126264. doi: 10.1016/j.biortech.2021.126264. Epub 2021 Nov 2.
The complex structure of lignocellulosic biomass forms the recalcitrance to prevent the embedded holo-cellulosic sugars from undergoing the biodegradation. Therefore, a pretreatment is often required for an efficient enzymatic lignocellulosic hydrolysis. Recently, glycerol organosolv (GO) pretreatment is revealed potent in selective deconstruction of various lignocellulosic biomass and effective improvement of enzymatic hydrolysis. Evidently, the GO pretreatment is capable to modify the structure of dissolved components by glycerolysis, i.e., by trans-glycosylation onto glyceryl glycosides and by hydroxylation grafting onto glyceryl lignin. Such modifications tend to protect these main components against excessive degradation, which can be mainly responsible for the obviously less fermentation inhibitors arising in the GO pretreatment. This pretreatment can provide opportunities for valorization of emerging lignocellulosic biorefinery with production of value-added biochemicals. Recent advances in GO pretreatment of lignocellulosic biomass followed by enzymatic hydrolysis are reviewed, and perspectives are made for addressing remaining challenges.
木质纤维素生物质的复杂结构形成了抗降解性，以防止嵌入的全细胞糖进行生物降解。因此，通常需要进行预处理以实现有效的酶法木质纤维素水解。最近，甘油有机溶剂（GO）预处理在各种木质纤维素生物质的选择性解构和酶解效率的提高方面显示出强大的作用。显然，GO 预处理能够通过甘油解改变溶解成分的结构，即通过向甘油基糖苷的转糖苷化和向甘油基木质素的羟化接枝。这种修饰倾向于保护这些主要成分免受过度降解，这可能是 GO 预处理中产生的发酵抑制剂明显较少的主要原因。这种预处理为新兴的木质纤维素生物炼制厂的增值生物化学产品的生产提供了机会。综述了 GO 预处理木质纤维素生物质及其随后的酶解的最新进展，并对解决剩余挑战提出了展望。
