多胺与蛋白质合成：对大肠杆菌各种需要多胺的突变体的研究。

Polyamines and protein synthesis: studies in various polyamine-requiring mutants of Escherichia coli.

作者信息

Goldemberg S H, Algranati I D

出版信息

Mol Cell Biochem. 1977 Jul 5;16(2):71-7. doi: 10.1007/BF01732046.

DOI:10.1007/BF01732046

Abstract

Different Escherichia coli mutants auxotrophic for polyamines were studied in order to investigate the relationships among polypeptide synthesis in cell-free systems, ribosomal distribution profiles and endogenous polyamine pools. The in vitro protein synthetic activity and the polyribosomal content were reduced in extracts from putrescine-starved cells of the double mutans MA 255 and MA 261, but not in the arginine-conditional auxotroph DK 6. Putrescine addition to the cultures of all these strains previously starved for polyamines, provoked a shift towards monomers in the equilibrium involving ribosomal particles. Concomitant changes in the intracellular levels of polyamines were observed: putrescine and spermidine increased markedly, and cadaverine disappeared.

摘要

为了研究无细胞系统中的多肽合成、核糖体分布图谱和内源性多胺库之间的关系，对不同的多胺营养缺陷型大肠杆菌突变体进行了研究。双突变体MA 255和MA 261的腐胺饥饿细胞提取物中的体外蛋白质合成活性和多核糖体含量降低，但精氨酸条件营养缺陷型DK 6的提取物中没有降低。向所有这些先前缺乏多胺的菌株培养物中添加腐胺，会导致核糖体颗粒平衡向单体方向转变。同时观察到细胞内多胺水平的变化：腐胺和亚精胺显著增加，尸胺消失。