多胺与抗生素对翻译的影响。

Polyamines and antibiotic effects on translation.

作者信息

Goldemberg S H, Algranati I D

出版信息

Med Biol. 1981 Dec;59(5-6):360-7.

Abstract

Protein synthesis was studied in polyamine-auxotrophic mutants of Escherichia coli. The decreased protein synthesizing rate observed both in vivo and in vitro in polyamine-starved bacteria is due to defective 30S ribosomal subparticles which are impaired in the initiation step of translation. Analysis of peptides synthesized in vivo suggests a more extensive misreading by putrescine-depleted bacteria. Cells grown without polyamine show a markedly decreased response to the inhibitory effect and the misreading inducing action of streptomycin in vivo and in vitro. The polyamine-starved bacteria are also less sensitive to other aminoglycoside antibiotics such as neomycin, kanamycin and kasugamycin.

摘要

对大肠杆菌的多胺营养缺陷型突变体中的蛋白质合成进行了研究。在多胺饥饿的细菌中，体内和体外观察到的蛋白质合成速率下降是由于30S核糖体亚颗粒有缺陷，其在翻译起始步骤中受损。对体内合成的肽段进行分析表明，腐胺缺乏的细菌存在更广泛的错读现象。在没有多胺的情况下生长的细胞，在体内和体外对链霉素的抑制作用和错读诱导作用的反应明显降低。多胺饥饿的细菌对其他氨基糖苷类抗生素（如新霉素、卡那霉素和春日霉素）也不太敏感。