Sawadogo Wamtinga Richard, Luo Yun, Elkington Bethany, He Tong-Chuan, Wang Chong-Zhi, Yuan Chun-Su
Tang Center for Herbal Medicine Research, Pritzker School of Medicine, University of Chicago, Chicago, Illinois, 60637, USA.
Department of Anesthesia and Critical Care, Pritzker School of Medicine, University of Chicago, Chicago, Illinois, 60637, USA.
Int J Appl Biol Pharm. 2020;11(3):170-187. Epub 2020 Aug 7.
(Vatke) Verdc. (Verbenaceae) is a seasonal herb widely spread in the West African region. The whole plant is used for the treatment of wounds, infections, and inflammatory pathologies. The purpose of this research is to evaluate the cytotoxicity and to analyze the probable pro-apototic, and cell cycle arrest effects of methylene chloride extract and its fractions against HCT-116 and HT-29 colorectal cancer cells using preliminary tests in order to highlight the interest of this plant in the search of new anticancer molecules. The dried powder of the whole plant was extracted by methylene chloride maceration for 24 hours and the extract was divided into five fractions. The cytotoxicity of the crude extract and fractions were evaluated by the MTS assay. The most active fractions were subjected to some preliminary assays including crystal violet, Hoechst staining, cell cycle arrest, and annexin V/PI assays on the cancer cells to highlight the probable mechanism of action of these fractions. The methylene chloride, ethyl acetate, and 1-butanol fractions of crude extract demonstrated significant antiproliferative effects on HCT-116 and HT-29 cell growth with IC values ranging between 2 to 15 μg/mL. 1-butanol and ethyl acetate fractions decreased the G1 phase by 20.53% and 28.47% and increased the G2/M by 23.47% and 25.90% respectively on HCT-116. Moreover, 1-butanol fraction increased the cumulative value of apoptotic cells by 49.77% on HCT-116 and ethyl acetate fraction increased this value by 53.37% at 15 μg/mL after 48 hours of exposure. The outcome of this study suggests the potential of 1-butanol and ethyl acetate fractions for the isolation of anticancer molecules against colorectal cancer.
（瓦泰克）马鞭草（马鞭草科）是一种广泛分布于西非地区的季节性草本植物。全株用于治疗伤口、感染和炎症性疾病。本研究的目的是通过初步试验评估二氯甲烷提取物及其馏分对HCT - 116和HT - 29结肠癌细胞的细胞毒性，并分析其可能的促凋亡和细胞周期阻滞作用，以突出该植物在寻找新的抗癌分子方面的价值。全株干燥粉末用二氯甲烷浸渍提取24小时，提取物分为五个馏分。通过MTS法评估粗提物和馏分的细胞毒性。对最具活性的馏分进行了一些初步试验，包括对癌细胞进行结晶紫染色、Hoechst染色、细胞周期阻滞和膜联蛋白V/PI检测，以突出这些馏分可能的作用机制。粗提物的二氯甲烷、乙酸乙酯和正丁醇馏分对HCT - 116和HT - 29细胞生长表现出显著的抗增殖作用，IC值在2至15μg/mL之间。在HCT - 116细胞上，正丁醇和乙酸乙酯馏分分别使G1期减少20.53%和28.47%，使G2/M期增加23.47%和25.90%。此外，在暴露48小时后，15μg/mL的正丁醇馏分使HCT - 116细胞凋亡细胞的累积值增加49.77%，乙酸乙酯馏分使该值增加53.37%。本研究结果表明，正丁醇和乙酸乙酯馏分具有分离抗结肠直肠癌抗癌分子的潜力。
