Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Department of Prosthodontics, School of Dentistry, University of Southern Santa Catarina, Tubarão, Santa Catarina, Brazil.
Microsc Res Tech. 2021 Feb;84(2):305-312. doi: 10.1002/jemt.23589. Epub 2020 Sep 11.
Root canal filling aims at eliminating empty spaces into the root canal system using biologically compatible materials. Three-dimensional root canal obturation must prevent or minimize the reinfection caused by microorganisms' leakage. This study aimed at evaluating whether fluorophore (Rhodamine or Fluo-3) influences the CLSM images of intratubular penetration of four endodontic sealers. Eighty bovine teeth were prepared using K files up to a size #70 and irrigated with 2.5% sodium hypochlorite. All roots were divided into eight groups (n = 10) according to the sealer and fluorophore used: AH Plus/Rhodamine, AH Plus/Fluo-3, Sealer Plus/Rhodamine, Sealer Plus/Fluo-3, Sealer Plus BC/Rhodamine, Sealer Plus BC/Fluo-3, Endosequence/Rhodamine, and Endosequence/Fluo-3. All roots were filled using cold lateral compaction technique. After 7 days, the roots were transversely sectioned, and three slices, one of each canal third, were obtained. Intratubular penetration was evaluated using CLSM. Sealer Plus BC/Rhodamine and Endosequence BC/Rhodamine presented higher intratubular penetration than AH Plus/Fluo-3 and Sealer Plus/Fluo-3 (p ˂ .05). The intragroup analysis showed similar intratubular penetration, regardless of the root third, except for the apical third in AH Plus/Fluo-3 and Sealer Plus BC/Fluo-3 groups. The type of fluorophore influences the calcium silicate sealers' tubular penetration but not of epoxy resin-based ones using CLSM. Bioceramic sealers should not be used associated with Rhodamine for CLSM evaluation. RESEARCH HIGHLIGHTS: The type of fluorophore influences the calcium silicate sealers' tubular penetration but not of epoxy resin-based ones when CLSM is used for assessment. Bioceramic sealers should not be used associated with Rhodamine.
根管充填的目的是使用生物相容性材料填充根管系统中的空腔。三维根管封闭必须防止或最小化微生物泄漏引起的再感染。本研究旨在评估荧光染料（若丹明或 Fluo-3）是否会影响四种根管封闭剂的管内渗透的 CLSM 图像。80 颗牛牙用 K 锉扩至 #70 号，并用 2.5%次氯酸钠冲洗。所有根部分为 8 组（n=10），根据使用的封闭剂和荧光染料进行分组：AH Plus/若丹明、AH Plus/Fluo-3、Sealer Plus/若丹明、Sealer Plus/Fluo-3、Sealer Plus BC/若丹明、Sealer Plus BC/Fluo-3、Endosequence/若丹明和 Endosequence/Fluo-3。所有根管均采用冷侧方加压技术充填。7 天后，将根管横向切割，每个根管的三分之一各取一个切片。使用 CLSM 评估管内渗透。Sealer Plus BC/若丹明和 Endosequence BC/若丹明的管内渗透高于 AH Plus/Fluo-3 和 Sealer Plus/Fluo-3（p<0.05）。组内分析显示，除了 AH Plus/Fluo-3 和 Sealer Plus BC/Fluo-3 组的根尖三分之一外，无论根管的哪一部分，都有相似的管内渗透。使用 CLSM 评估时，荧光染料的类型会影响钙硅酸钠封闭剂的管内渗透，但不会影响基于环氧树脂的封闭剂。生物陶瓷封闭剂不应与若丹明一起用于 CLSM 评估。研究亮点：当使用 CLSM 进行评估时，荧光染料的类型会影响钙硅酸钠封闭剂的管内渗透，但不会影响基于环氧树脂的封闭剂。生物陶瓷封闭剂不应与若丹明一起使用。
