从一名具有复原力的阿尔茨海默病患者中生成并鉴定一种新型人类诱导多能干细胞系。
Generation and characterization of a novel human iPSC line from a resilient Alzheimer's disease patient.
作者信息
Das Debamitra, Li Jiaxin, Liu Senquan, Oh Esther, Cheng Linzhao, Lyketsos Constantine, Mahairaki Vasiliki
机构信息
Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
Division of Hematology, Department of Medicine, and the Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore MD 21287, USA.
出版信息
Stem Cell Res. 2020 Oct;48:101979. doi: 10.1016/j.scr.2020.101979. Epub 2020 Sep 2.
Alzheimer's disease (AD) is a progressive neurodegenerative disease that is the major cause of dementia in older people. Here, we report the derivation of human induced pluripotent stem cells (iPSCs) from an AD patient at age of 80 who has the APOE ε4/ε4 genotype and is resilient to cognitive decline for 10 years. The iPSCs reprogrammed from the blood cells of this patient by transient expression of pluripotency genes maintain the ε4/ε4 genotype, are karyotypically normal and display typical iPSC characteristics. Upon differentiation, the iPSCs are able to differentiate into cells of the three germ layers, confirming their pluripotency.
阿尔茨海默病（AD）是一种进行性神经退行性疾病，是老年人痴呆的主要原因。在此，我们报告了从一名80岁的AD患者身上获得人诱导多能干细胞（iPSC），该患者具有APOE ε4/ε4基因型，且在10年里对认知衰退具有抵抗力。通过多能性基因的瞬时表达从该患者血细胞重编程得到的iPSC保持了ε4/ε4基因型，核型正常，并表现出典型的iPSC特征。分化后，iPSC能够分化为三个胚层的细胞，证实了它们的多能性。
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