建立SIAISi002 - A诱导多能干细胞（iPSC）系，该细胞系来自一名39岁有阿尔茨海默病（AD）家族病史且携带两份APOE4基因的健康女性供体。
Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene.
作者信息
Yan Yi, Qiu Yinghui, Zhao Aonan, Zhao Jian, Wang Ying, Deng Yulei
机构信息
Department of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
出版信息
Stem Cell Res. 2020 May;45:101792. doi: 10.1016/j.scr.2020.101792. Epub 2020 Apr 22.
A 39-year old healthy female with a family history of Alzheimer's disease (AD) and two copies of apolipoprotein E(APOE) ε4 allele donated her Peripheral blood mononuclear cells (PBMC). The non-integrative Sendai viral vectors used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers. Furthermore, the iPSC line displayed a normal karyotype. The APOE gene is currently considered to be the most relevant gene for sporadic AD. Our model might offer a platform to study the pathological mechanisms and drug testing studies in AD.
一名39岁、有阿尔茨海默病（AD）家族史且携带两份载脂蛋白E（APOE）ε4等位基因拷贝的健康女性捐赠了她的外周血单个核细胞（PBMC）。使用非整合型仙台病毒载体，利用人类OKSM转录因子对PBMC进行重编程。通过免疫细胞化学以及诱导多能干细胞（iPSC）自发分化为三个胚层的能力，证实了iPSC的多能性。此外，该iPSC系显示出正常的核型。APOE基因目前被认为是散发性AD最相关的基因。我们的模型可能为研究AD的病理机制和药物测试研究提供一个平台。
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