Jayaraj A P
Department of Surgery, University College London, UK.
Br J Exp Pathol. 1988 Jun;69(3):379-86.
The process of collagenolysis and the source of collagenase liberated from different cell types in the colonic mucosa has been investigated by the lysis of collagen gels in vitro. The reconstituted collagen gel strongly reacted to periodic acid Schiff (PAS) when stained with combined alcian blue-PAS, indicating the presence of glycoprotein with neutral sugars in the collagen gel. Colonic explants of rabbits produced visible collagenolysis. An area of alcian blue stained gel was seen replacing the usual PAS staining around the area of the lysis. Several histochemical methods revealed that the columnar cells had multiplied with high enzymatic activity and penetrated the collagen gel where collagenolysis took place. The action of several proteolytic enzymes on collagen gel showed that ficin caused lytic activity, even though collagen is resistant to most proteolytic enzymes. Papain, pepsin and trypsin altered composition of collagen gel from neutral mucopolysaccharide to acid mucopolysaccharide. Collagenase and pronase at low concentration were found to cause extensive collagenolysis. The synthesis and breakdown of collagen is a desirable balanced process in the remodelling of connective tissue. This dynamic equilibrium may be achieved through the subtle interplay of cells liberating and inhibiting collagenase.
通过体外胶原凝胶的溶解，研究了结肠黏膜中胶原溶解的过程以及不同细胞类型释放的胶原酶的来源。用阿尔辛蓝 - 过碘酸希夫（PAS）联合染色时，重构的胶原凝胶对PAS有强烈反应，表明胶原凝胶中存在带有中性糖的糖蛋白。兔结肠外植体产生了明显的胶原溶解。在溶解区域周围，可见阿尔辛蓝染色的凝胶区域取代了通常的PAS染色。几种组织化学方法显示，柱状细胞大量增殖且酶活性高，并穿透发生胶原溶解的胶原凝胶。几种蛋白水解酶对胶原凝胶的作用表明，尽管胶原对大多数蛋白水解酶具有抗性，但无花果蛋白酶仍能引起溶解活性。木瓜蛋白酶、胃蛋白酶和胰蛋白酶使胶原凝胶的成分从中性粘多糖转变为酸性粘多糖。发现低浓度的胶原酶和链霉蛋白酶会引起广泛的胶原溶解。在结缔组织重塑过程中，胶原的合成和分解是一个理想的平衡过程。这种动态平衡可能通过释放和抑制胶原酶的细胞之间的微妙相互作用来实现。
