West M J, Coleman P D, Flood D G
Institute of Anatomy B, University of Aarhus, Denmark.
Brain Res. 1988 May 10;448(1):167-72. doi: 10.1016/0006-8993(88)91114-6.
A practical example is given of how a newly developed stereological estimator of particle number, the disector, can be used to make estimates of neuron number in the dentate gyrus of rats. The estimates are free of biases related to lost caps, overprojection and assumptions about size, shape and orientation of the objects that are counted. The disector principle and the practical considerations relating to histological preparations and sampling are presented.
文中给出了一个实际例子，说明新开发的粒子数量体视学估计器—— dissector，如何用于估计大鼠齿状回中的神经元数量。这些估计不受与丢失帽、过度投射以及对所计数物体的大小、形状和方向的假设相关的偏差影响。文中介绍了dissector原理以及与组织学标本制备和采样相关的实际注意事项。
