Department of Microbiology and immunology, School of Health Sciences Center, Xi'an Jiaotong University, Xi'an, China; Department of Stomatology, the First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.
Stomatology Department, Xi'an Gaoxin Hospital, Xi'an, China.
Microb Pathog. 2020 Dec;149:104479. doi: 10.1016/j.micpath.2020.104479. Epub 2020 Sep 11.
Liver disease has been reported to associate with oral microbiota. This study aimed to identify the salivary microbial structure in liver disease patients and determine whether the disease progression influence the bacterial composition. 16S rDNA high-throughput sequencing and bioinformatic analysis were used to examine oral bacterial diversity in the different status of hepatitis patients including 6 patients with Hepatitis B (Y), 6 patients with Hepatitis B Cirrhosis (YY) and 6 patients with liver cancer (C), and 6 healthy controls (T). Phylogenetic analysis revealed that the genera of Streptococcus, Prevotella, Actinomyces, Veillonella and Neisseria are predominant genus in the saliva of Y, YY, C patients and T group. Lautropia, Abiotrophia and Veillonella were enriched in Y patients, while Treponema, Selenomonas and Oribacterium were also existed in YY patients. Haemophilus, Porphyromonas and Filifactor had high abundance in C patients. The genera of Moryella, Leptotrichia, Lactobacillus, Dialister, Serratia, Enterococcus and Actinobacillus were decreased in all patient samples compared with healthy control samples which may be used for treatment of liver disease. Diversity analyses showed decreased diversity of salivary bacterial communities was discovered in the progress of the liver disease. These findings identified the oral microbiota dysbiosis in liver disease, which may providing available information and possible diagnostic biomarkers for liver patients.
肝病已被报道与口腔微生物群有关。本研究旨在确定肝病患者的唾液微生物结构，并确定疾病进展是否影响细菌组成。使用 16S rDNA 高通量测序和生物信息学分析来检查不同肝炎患者（包括 6 名乙型肝炎患者（Y）、6 名乙型肝炎肝硬化患者（YY）和 6 名肝癌患者（C）和 6 名健康对照者（T））口腔细菌多样性。系统发育分析显示，链球菌属、普雷沃氏菌属、放线菌属、韦荣球菌属和奈瑟菌属是 Y、YY、C 患者和 T 组唾液中的主要属。乳杆菌属、嗜血杆菌属、拟杆菌属和卟啉单胞菌属在 C 患者中含量较高。Lautropia、Abiotrophia 和 Veillonella 在 Y 患者中富集，而 Treponema、Selenomonas 和 Oribacterium 也存在于 YY 患者中。Moryella、Leptotrichia、Lactobacillus、Dialister、Serratia、Enterococcus 和 Actinobacillus 等属在所有患者样本中均低于健康对照组样本，这可能用于治疗肝病。多样性分析表明，在肝病进展过程中，唾液细菌群落的多样性降低。这些发现确定了肝病中的口腔微生物失调，这可能为肝病患者提供了有价值的信息和可能的诊断生物标志物。
