Chen Li-Jun, Xu Wang, Li Ya-Ping, Ma Li-Ting, Zhang Hui-Fang, Huang Xiao-Bo, Yu Geng-Geng, Ma Xiu-Qin, Chen Chao, Liu Yan-Hong, Wu Jie, Wang Li-Jun, Xu Yuan
Department of Respiratory and Critical Medicine, The First People's Hospital of Yinchuan City, Yinchuan, Ningxia, People's Republic of China.
Department of Respiratory and Critical Medicine, The Second Affiliated Hospital of Ningxia Medical University, Yinchuan, Ningxia, People's Republic of China.
Int J Chron Obstruct Pulmon Dis. 2020 Aug 24;15:1997-2004. doi: 10.2147/COPD.S254172. eCollection 2020.
Chronic obstructive pulmonary disease (COPD) is a chronic airway inflammatory disease characterized by irreversible airflow obstruction. Pathogenic mechanisms underlying COPD remain largely unknown.
The current study was designed to explore serum concentration of hypoxia-inducible factor 1α (HIF-1α) in stable COPD patients and the potential effect of polysaccharides (LBP) on HIF-1α protein expression.
Serum HIF-1α was quantified by ELISA in 102 stable COPD patients before and after 2-week orally taken LBP (100 mL/time, twice daily, 5-15 mg/mL). Correlation of serum LBP and lung function (FEV1%) or blood gas (PO and PCO) was also analyzed. As a control, 105 healthy subjects were also enrolled into this study.
Serum concentration of HIF-1α was significantly higher in the stable COPD patients (37.34 ± 7.20 pg/mL) than that in the healthy subjects (29.55 ± 9.66 pg/mL, <0.001). Oral administration of LBP (5 mg/mL, 100 mL, twice daily for 2 weeks) not only relieved COPD symptoms but also significantly reduced serum HIF-1α concentration (36.94 ± 9.23 vs 30.49 ± 6.42 pg/mL, <0.05). In addition, level of serum HIF-1α concentration was significantly correlated with PCO (r = 0.283, <0.001), but negatively and significantly correlated with PO (r = -0.490, =0.005) or FEV1%(r = -0.420, =0.018).
These findings suggested that activation of HIF-1 signaling pathway may be involved in the pathophysiology of COPD and that stabilization of serum HIF-1α concentration by LBP might benefit the stable COPD patients.
慢性阻塞性肺疾病（COPD）是一种以不可逆气流受限为特征的慢性气道炎症性疾病。COPD的发病机制在很大程度上仍不清楚。
本研究旨在探讨稳定期COPD患者血清缺氧诱导因子1α（HIF-1α）浓度以及枸杞多糖（LBP）对HIF-1α蛋白表达的潜在影响。
采用酶联免疫吸附测定法（ELISA）对102例稳定期COPD患者口服LBP（100 mL/次，每日2次，5 - 15 mg/mL）2周前后的血清HIF-1α进行定量分析。同时分析血清LBP与肺功能（FEV1%）或血气指标（PO₂和PCO₂）的相关性。作为对照，本研究还纳入了105名健康受试者。
稳定期COPD患者血清HIF-1α浓度（37.34 ± 7.20 pg/mL）显著高于健康受试者（29.55 ± 9.66 pg/mL，P < 0.001）。口服LBP（5 mg/mL，100 mL，每日2次，共2周）不仅缓解了COPD症状，还显著降低了血清HIF-1α浓度（36.94 ± 9.23 vs 30.49 ± 6.42 pg/mL，P < 0.05）。此外，血清HIF-1α浓度水平与PCO₂显著正相关（r = 0.283，P < 0.001），但与PO₂（r = -0.490，P = 0.005）或FEV1%（r = -0.420，P = 0.018）显著负相关。
这些研究结果表明，HIF-1信号通路的激活可能参与了COPD的病理生理过程，LBP稳定血清HIF-1α浓度可能对稳定期COPD患者有益。
