Akhrem A A, Tishchenko E I, Kiselev P A, Metelitsa D I
Biokhimiia. 1978 Nov;43(11):2033-7.
At 20 degrees C, in a phosphate buffer, pH 5,8--8,0, methanol and aniline interactions with hemoglobin and cytochrome c were studied using the difference spectrophotometry method. The difference absorption spectra are characterized by following values of lambdamax and lambdamin (nm): I--MeOH--hemoglobin (405 and 420), II-MeOH--cytochrome c (405--406 and 419--422), III--aniline--cytochrome c (421--410 and 401--396). The values of lambdamax and lambdamin for system III are shifted in the region of shorter wavelengths from 421 to 410 nm and from 401 to 396 nm, respectively within the pH range of 5,8--7,95. From difference spectra for systems I, II, III the dissociation constants of complexes obtained, Ks were calculated. Log Ks is linearly dependent on pH. System I is characterized by two values of Ks at all pH. The Ks values were calculated in general form from the dependences obtained. The nature of the complexes is discussed.
在20摄氏度下，于pH值为5.8 - 8.0的磷酸盐缓冲液中，使用差示分光光度法研究了甲醇和苯胺与血红蛋白及细胞色素c的相互作用。差示吸收光谱的特征在于以下最大波长（λmax）和最小波长（λmin）值（纳米）：I - 甲醇 - 血红蛋白（405和420），II - 甲醇 - 细胞色素c（405 - 406和419 - 422），III - 苯胺 - 细胞色素c（421 - 410和401 - 396）。在5.8 - 7.95的pH范围内，系统III的λmax和λmin值分别在较短波长区域从421纳米移至410纳米以及从401纳米移至396纳米。根据系统I、II、III的差示光谱，计算了所得配合物的解离常数Ks。log Ks与pH呈线性相关。系统I在所有pH值下均具有两个Ks值。Ks值根据所得依赖关系以一般形式计算得出。对配合物的性质进行了讨论。
