Fan Zhaoxuan, Feng Xiao, Zhang Weifei, Zhang Xueji, Lin Jin-Ming
Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China.
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China.
J Pharm Anal. 2020 Aug;10(4):329-333. doi: 10.1016/j.jpha.2020.04.003. Epub 2020 Apr 14.
Researches on detection of human papillomavirus (HPV) high-risk samples were carried out by polymerase chain reaction (PCR) coupled with microchip electrophoresis (MCE). Herein, we introduced a simple, rapid, automated method for detecting high-risk samples HPV16 and HPV18. In this research, general primers were initially selected to obtain sufficient detectable yield by PCR to verify feasibility of MCM method for HPV detection, then type-specific primers were further used to evaluate the specificity of MCE method. The results indicated MCE method was capable of specifically detecting high-risk HPV16 and HPV18, and also enabled simultaneous detection of multiplex samples. This MCE method described here has been successfully applied to HPV detection and displayed excellent reliability demonstrating by sequencing results. The inherent capability of MCE facilitated HPV detection conducted in a small chip with automated, high throughput, massive parallelized analysis. We envision that MCE method will definitely pave a way for clinical diagnosis, and even on-site screening of cervical cancer.
通过聚合酶链反应（PCR）结合微芯片电泳（MCE）对人乳头瘤病毒（HPV）高危样本进行检测研究。在此，我们介绍了一种简单、快速、自动化的检测高危样本HPV16和HPV18的方法。在本研究中，首先选择通用引物通过PCR获得足够的可检测产量，以验证MCM方法用于HPV检测的可行性，然后进一步使用型特异性引物评估MCE方法的特异性。结果表明，MCE方法能够特异性检测高危HPV16和HPV18，还能够同时检测多个样本。这里描述的这种MCE方法已成功应用于HPV检测，并通过测序结果显示出优异的可靠性。MCE的内在能力有助于在小芯片中进行HPV检测，实现自动化、高通量、大规模平行分析。我们设想，MCE方法必将为宫颈癌的临床诊断乃至现场筛查铺平道路。
