Podolsky D K, Fournier D A
Department of Medicine, Harvard Medical School, Boston, Massachusetts.
Gastroenterology. 1988 Aug;95(2):379-87. doi: 10.1016/0016-5085(88)90494-5.
The presence of several glycoconjugates in colonic mucosa of patients with inflammatory bowel disease was assessed through indirect immunofluorescent staining using a collection of 23 monoclonal antibodies directed against human colonic mucin glycoproteins (anti-HCM MAbs). Intensity and distribution of staining by three anti-HCM MAbs were significantly altered in mucosa from patients with ulcerative colitis (UC) (n = 14) when compared with normal tissue (n = 15) and with tissue from patients with Crohn's disease as well as other inflammatory disorders (n = 15). Staining by anti-HCM MAb 17, which binds to colonic mucin glycoprotein species IV and V, was absent or diminished in 86% of samples from patients with active UC in contrast to 14% of normal and disease control specimens. Reduction in anti-HCM MAb 17 staining was less marked in mucosal biopsy specimens from patients with UC lacking acute inflammatory activity (n = 8). In contrast to the apparent loss of the MAb 17-defined epitopes, staining with anti-HCM MAbs 10 and 22 was enhanced in UC tissue compared with normal and disease controls. Increased staining with MAb 10 was present in 93% of samples from UC patients demonstrating active inflammation. Increased MAb 10 staining was not apparent in noninvolved mucosa from UC patients, indicating that increased expression of the specified epitope is related to the acute inflammatory process. In contrast, indirect immunofluorescent staining with MAb 22 was apparent in both involved (78%) and uninvolved (67%) UC mucosa in contrast to normal and disease controls (less than 12%). In addition, staining with several other anti-HCM MAbs (MAbs 3, 11, 15) was modestly and variably diminished (14%-28%) in UC, Crohn's disease, and other inflammatory disorders. These findings demonstrate the presence of alterations in mucosal content of specific glycoconjugate structures in association with UC. Inflammatory processes may also result in broad changes in glycoconjugate determinants generally.
通过间接免疫荧光染色，使用一组针对人结肠粘蛋白糖蛋白的23种单克隆抗体（抗人结肠粘蛋白单克隆抗体），评估炎症性肠病患者结肠黏膜中几种糖缀合物的存在情况。与正常组织（n = 15）以及克罗恩病和其他炎症性疾病患者的组织（n = 15）相比，溃疡性结肠炎（UC）患者（n = 14）黏膜中三种抗人结肠粘蛋白单克隆抗体的染色强度和分布有显著改变。与14%的正常和疾病对照标本相比，与结肠粘蛋白糖蛋白IV和V结合的抗人结肠粘蛋白单克隆抗体17在86%的活动期UC患者样本中染色缺失或减弱；在缺乏急性炎症活动的UC患者黏膜活检标本（n = 8）中，抗人结肠粘蛋白单克隆抗体17染色的减少不太明显。与单克隆抗体17定义的表位明显缺失相反，与正常和疾病对照相比，UC组织中抗人结肠粘蛋白单克隆抗体10和22的染色增强。在93%表现出活动炎症的UC患者样本中，单克隆抗体10染色增加；在UC患者未受累的黏膜中，单克隆抗体10染色增加不明显，这表明特定表位表达的增加与急性炎症过程有关。相比之下，与正常和疾病对照（低于12%）相比，单克隆抗体22的间接免疫荧光染色在UC受累黏膜（78%）和未受累黏膜（67%）中均明显。此外，在UC、克罗恩病和其他炎症性疾病中，几种其他抗人结肠粘蛋白单克隆抗体（单克隆抗体3、11、15）的染色适度且有变化地减弱（14%-28%）。这些发现表明，与UC相关的特定糖缀合物结构的黏膜含量存在改变。炎症过程也可能导致糖缀合物决定簇普遍发生广泛变化。
