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从农田分离的细菌物种对选定病原体的抗菌活性及分子特征

antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens.

作者信息

Onajobi Ismail B, Idowu Esther O, Adeyemi Jamiu O, Samson Oyindamola J, Ogunyinka Peter I, Fagade Obasola E

机构信息

Department of Microbiology, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.

Department of Mathematical Sciences, Faculty of Science, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.

出版信息

Biotechnol Rep (Amst). 2020 Aug 9;27:e00513. doi: 10.1016/j.btre.2020.e00513. eCollection 2020 Sep.

Abstract

This study aims to screen bacterial isolates from Olabisi Onabanjo University Farmland for antibacterial activity against pathogenic microorganisms. Agar well diffusion method was used. Isolates were identified molecularly. Chi-square test revealed significant association between isolates, antibacterial activity with likelihood -value = 0.000 and 5% significant level. Six among thirty-five isolates exhibited antibacterial activity against the test pathogenic species. A greater antibacterial activity (50 % inhibition) was observed in strain PRE16. It inhibited the growth of and by 23.00 ± 2.00, 18.00 ± 2.00 and 20.00 ± 4.00 respectively. DNA sequencing revealed antagonist isolates as sp. BCN2, strain P30C4, strain KNUC205, strain KEM24 strain CSD05 and strain PRE16. Phylogenetic analysis revealed close relatedness of most isolates with species strains. These strains are suggested to be effective for the discovery of new antibacterial agents.

摘要

本研究旨在从奥拉比西·奥纳班乔大学农田中筛选出对致病微生物具有抗菌活性的细菌分离株。采用琼脂扩散法。通过分子方法对分离株进行鉴定。卡方检验显示分离株、抗菌活性之间存在显著关联,似然值 = 0.000,显著性水平为5%。35株分离株中有6株对受试致病菌种表现出抗菌活性。在PRE16菌株中观察到更强的抗菌活性(50%抑制)。它分别抑制金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的生长达23.00±2.00、18.00±2.00和20.00±4.00。DNA测序显示拮抗性分离株为芽孢杆菌属sp. BCN2、假单胞菌属菌株P30C4、芽孢杆菌属菌株KNUC205、芽孢杆菌属菌株KEM24、芽孢杆菌属菌株CSD05和芽孢杆菌属菌株PRE16。系统发育分析显示大多数分离株与芽孢杆菌属菌株密切相关。这些菌株被认为对发现新型抗菌剂有效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f7e/7475235/d2a8ddc908b5/gr1.jpg

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