Department of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju, Gyeongbuk 37224, Republic of Korea.
Plant Dis. 2020 Nov;104(11):2866-2874. doi: 10.1094/PDIS-01-20-0050-RE. Epub 2020 Sep 14.
Apple fruits with anthracnose symptoms were collected from commercial apple orchards in different regions of the Republic of Korea, and isolations were made on potato dextrose agar to isolate the causal agents. The fungal isolates were identified based on their morphological characteristics, growth rates, and multigene sequences. Nine isolates were identified via phylogenetic analysis: three , two . , one . (s.s.), two . , and one . isolates. The pathogenicity of the isolates was tested using detached apple fruits under laboratory conditions. This study also reidentified six isolates responsible for apple anthracnose, which were deposited in the Korean Agricultural Culture Collection. Among the six isolates, three were identified as . (deposited as . s.s.), and three were . (deposited as . s.s.). All the species identified in this study were highly sensitive to tebuconazole in terms of inhibition of mycelial growth (EC value of 0.12 to 2.1 μg/ml).
从韩国不同地区的商业苹果园中采集到有炭疽病症状的苹果果实，并在土豆葡萄糖琼脂上进行分离，以分离致病因子。根据形态特征、生长速度和多基因序列对真菌分离物进行鉴定。通过系统发育分析鉴定出 9 个分离物：3 个. ，2 个. ，1 个. （s.s.），2 个. 和 1 个. 分离物。在实验室条件下使用离体苹果果实测试了 分离物的致病性。本研究还重新鉴定了 6 个引起苹果炭疽病的 分离物，这些分离物已被保藏在韩国农业文化收藏中。在这 6 个分离物中，有 3 个被鉴定为. （保藏为. s.s.），3 个为. （保藏为. s.s.）。本研究中鉴定的所有 种对三唑酮的抑菌生长均高度敏感（EC 值为 0.12 至 2.1 μg/ml）。
