Department of Ecology & Environmental System, College of Ecology & Environmental Sciences, Kyungpook National University, Sangju, Gyeongbuk, 37224, Republic of Korea.
Division of Life and Environmental Sciences, College of Life and Environmental Sciences, Daegu University, Gyeongsan, Gyeongbuk, 38453, Republic of Korea.
Sci Rep. 2019 Aug 19;9(1):12089. doi: 10.1038/s41598-019-48108-1.
A total of 24 Colletotrichum isolates were isolated from diseased Japanese plum (Prunus salicina) fruits showing chlorotic regions with whitish-brown sunken necrotic lesions and phylogenetic relationships among the collected Colletotrichum isolates were determined. A subset of 11 isolates was chosen for further taxonomic study based on morphology and molecular characteristics identified using the internal transcribed spacer (ITS) and beta-tubulin (TUB2) genes. Isolates in the C. acutatum complex were analyzed using partial sequencing of five gene regions (ITS, GAPDH, ACT, TUB2, and CHS), and C. gloeosporioides sensu lato (s.l.) isolates were analyzed using seven gene regions (ITS, TUB2, GAPDH, ACT, CAL, CHS-1, and ApMat). Morphological assessments in combination with phylogenetic analysis delineated four species of Colletotrichum including C. gloeosporioides sensu stricto (s.s.), C. nymphaeae, C. foriniae, and C. siamense; these data identify Colletotrichum fioriniae and C. siamense two new species associated with plum anthracnose in South Korea. Finally, the pathogenicity of these four species in the development of plum anthracnose in South Korea was confirmed by inoculations of plum fruit.
共从表现出褪绿区域和灰白色棕色凹陷坏死病斑的患病日本李（Prunus salicina）果实中分离到 24 个炭疽菌分离物，并确定了收集的炭疽菌分离物之间的系统发育关系。根据内部转录间隔区（ITS）和β-微管蛋白（TUB2）基因鉴定的形态和分子特征，选择了 11 个分离物进行进一步的分类学研究。使用五个基因区域（ITS、GAPDH、ACT、TUB2 和 CHS）对炭疽菌复合种中的分离物进行部分测序，并使用七个基因区域（ITS、TUB2、GAPDH、ACT、CAL、CHS-1 和 ApMat）对炭疽菌广义种（s.l.）中的分离物进行分析。形态评估与系统发育分析相结合，划定了包括炭疽菌严格意义上（s.s.）、C. nymphaeae、C. foriniae 和 C. siamense 在内的 4 种炭疽菌；这些数据确定了炭疽菌 fioriniae 和 C. siamense 是与韩国李炭疽病相关的两个新种。最后，通过对李果实的接种证实了这 4 个种在韩国李炭疽病发展中的致病性。
