Wang Jing, Wang Ping, He Ying, Liu Xiaoli, Wang Sisi, Ma Chunxing, Tian Xiaofei, Wang Jing, Wu Xin
Department of Gynaecology and Obstetrics, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei Province, China.
Life Science Research Center, Hebei North University, Zhangjiakou, Hebei Province, China.
Aging (Albany NY). 2020 Sep 14;12(17):17625-17633. doi: 10.18632/aging.103821.
To investigate the antitumor effects of Graphene oxide (GO) on tumor invasion and metastasis in human cervical cancer Hela cells.
GO significantly inhibited cell viability and the number of clones, promoted cell apoptosis, as well as suppressed cell migration and invasion, and destroyed the structure of actin cytoskeleton of Hela cells in a dose-dependent manner in. Moreover, the expression of metastasis-related proteins, including MMP2 and Cdc42, were significantly suppressed by the treatment of GO. And the expression of MMP3 was remarkably increased by Smad inhibitor and the protein levels of MMP3 and ICAM were elevated by the JNK inhibitor in GO-treated Hela cells.
GO exhibited inhibitory effects on cell migration and invasion possibly by destroying actin cytoskeleton in Hela cells, which is a potential component of the Smad and JNK signalling pathways.
GO was prepared and chracterized by UV visible light absorption spectroscopy and atomic force microscopy. Hela cells were treated with Go at different dose levels. Then, in vitro cytotoxicity of GO was evaluated by the MTT assay, colony-forming assay and cell apoptosis assay. The inhibitory effects of GO on tumor cell migration and invasion as well as actin cytoskeleton were explored using Hela cells.
研究氧化石墨烯（GO）对人宫颈癌Hela细胞肿瘤侵袭和转移的抗肿瘤作用。
GO显著抑制细胞活力和克隆数量，促进细胞凋亡，抑制细胞迁移和侵袭，并以剂量依赖的方式破坏Hela细胞的肌动蛋白细胞骨架结构。此外，GO处理显著抑制了包括MMP2和Cdc42在内的转移相关蛋白的表达。在GO处理的Hela细胞中，Smad抑制剂使MMP3的表达显著增加，JNK抑制剂使MMP3和ICAM的蛋白水平升高。
GO可能通过破坏Hela细胞中的肌动蛋白细胞骨架对细胞迁移和侵袭产生抑制作用，这是Smad和JNK信号通路的潜在组成部分。
通过紫外可见吸收光谱和原子力显微镜制备并表征GO。用不同剂量水平的GO处理Hela细胞。然后，通过MTT法、集落形成法和细胞凋亡法评估GO的体外细胞毒性。使用Hela细胞探索GO对肿瘤细胞迁移、侵袭以及肌动蛋白细胞骨架的抑制作用。
