Radiation Biology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt.
Petroleum Applications Department, Egyptian Petroleum Research Institute (EPRI), Cairo, Egypt.
Sci Rep. 2024 May 21;14(1):11535. doi: 10.1038/s41598-024-60347-5.
In this study, a novel method for the fabrication of hesperidin/reduced graphene oxide nanocomposite (RGOH) with the assistance of gamma rays is reported. The different RGOHs were obtained by varying hesperidin concentrations (25, 50, 100, and 200 wt.%) in graphene oxide (GO) solution. Hesperidin concentrations (25, 50, 100, and 200 wt.%) in graphene oxide (GO) were varied to produce the various RGOHs. Upon irradiation with 80 kGy from γ-Ray, the successful reduction of GO occurred in the presence of hesperidin. The reduction process was confirmed by different characterization techniques such as FTIR, XRD, HRTEM, and Raman Spectroscopy. A cytotoxicity study using the MTT method was performed to evaluate the cytotoxic-anticancer effects of arbitrary RGOH on Wi38, CaCo2, and HepG2 cell lines. The assessment of RGOH's anti-inflammatory activity, including the monitoring of IL-1B and IL-6 activities as well as NF-kB gene expression was done. In addition, the anti-invasive and antimetastatic properties of RGOH, ICAM, and VCAM were assessed. Additionally, the expression of the MMP2-9 gene was quantified. The assessment of apoptotic activity was conducted by the detection of gene expressions related to BCl2 and P53. The documentation of the JNK/SMAD4/MMP2 signaling pathway was ultimately accomplished. The findings of our study indicate that RGOH therapy has significant inhibitory effects on the JNK/SMAD4/MMP2 pathway. This suggests that it could be a potential therapeutic option for cancer.
在这项研究中，报道了一种在γ射线辅助下制备橙皮苷/还原氧化石墨烯纳米复合材料（RGOH）的新方法。通过改变氧化石墨烯（GO）溶液中橙皮苷的浓度（25、50、100 和 200wt.%），获得了不同的 RGOH。通过改变氧化石墨烯（GO）中的橙皮苷浓度（25、50、100 和 200wt.%），制备了不同的 RGOH。在 80kGyγ射线辐照下，在橙皮苷的存在下成功还原了 GO。通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、高分辨率透射电子显微镜（HRTEM）和拉曼光谱等不同表征技术证实了还原过程。通过 MTT 法进行细胞毒性研究，评估任意 RGOH 对 Wi38、CaCo2 和 HepG2 细胞系的细胞毒性-抗癌作用。监测 IL-1B 和 IL-6 活性以及 NF-kB 基因表达，评估 RGOH 的抗炎活性。此外，评估 RGOH 的抗侵袭和抗转移特性，包括 ICAM 和 VCAM 的表达。还定量了 MMP2-9 基因的表达。通过检测与 BCl2 和 P53 相关的基因表达，评估凋亡活性。最终记录了 JNK/SMAD4/MMP2 信号通路的情况。我们的研究结果表明，RGOH 治疗对 JNK/SMAD4/MMP2 通路具有显著的抑制作用。这表明它可能是癌症的一种潜在治疗选择。
