Won Hansol, Kim Sung-Hyun, Yang Jun-Young, Jung Kikyung, Jeong Jayoung, Oh Jae-Ho, Lee Jin-Hee
Division of Toxicological Research, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, 187, Osongsaengmyeong 2-Ro, Cheongju 28159, Korea.
Toxics. 2022 May 5;10(5):236. doi: 10.3390/toxics10050236.
The nano-market has grown rapidly over the past decades and a wide variety of products are now being manufactured, including those for biomedical applications. Despite the widespread use of nanomaterials in various industries, safety and health effects on humans are still controversial, and testing methods for nanotoxicity have not yet been clearly established. Nanomaterials have been reported to interfere with conventional cytotoxicity tests due to their unique properties, such as light absorption or light scattering. In this regard, the colony-forming efficacy (CFE) assay has been suggested as a suitable test method for testing some nanomaterials without these color-interferences. In this study, we selected two types of GNPs (Graphene nanoplatelets) as test nanomaterials and evaluated CFE assay to assess the cytotoxicity of GNPs. Moreover, for further investigation, including expansion into other cell types, GNPs were evaluated by the conventional cytotoxicity tests including the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), Cell Counting Kit-8 (CCK-8), and Neutral red uptake (NRU) assay using MDCK, A549 and HepG2 cells. The results of CFE assay suggest that this test method for three cell lines can be applied for GNPs. In addition, the CFE assay was able to evaluate cytotoxicity regardless more accurately of color interference caused by residual nanomaterials.
在过去几十年中，纳米市场迅速发展，现在正在生产各种各样的产品，包括用于生物医学应用的产品。尽管纳米材料在各个行业中广泛使用，但对人类的安全和健康影响仍存在争议，并且纳米毒性的测试方法尚未明确确立。据报道，纳米材料因其独特的性质，如光吸收或光散射，会干扰传统的细胞毒性测试。在这方面，集落形成效率（CFE）测定法已被建议作为一种合适的测试方法，用于测试一些没有这些颜色干扰的纳米材料。在本研究中，我们选择了两种类型的石墨烯纳米片（GNPs）作为测试纳米材料，并评估CFE测定法以评估GNPs的细胞毒性。此外，为了进一步研究，包括扩展到其他细胞类型，使用MDCK、A549和HepG2细胞通过传统的细胞毒性测试，包括3-(4,5-二甲基噻唑-2-基)-5-(3-羧甲氧基苯基)-2-(4-磺基苯基)-2H-四唑（MTS）、细胞计数试剂盒-8（CCK-8）和中性红摄取（NRU）测定法对GNPs进行评估。CFE测定法的结果表明，这种针对三种细胞系的测试方法可用于GNPs。此外，CFE测定法能够更准确地评估由残留纳米材料引起的颜色干扰对细胞毒性的影响。
