Lewis R M, Cosgriff T M, Griffin B Y, Rhoderick J, Jahrling P B
Medical Division and Disease Assessment Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701.
J Gen Virol. 1988 Jul;69 ( Pt 7):1735-9. doi: 10.1099/0022-1317-69-7-1735.
The U937 monocytic cell line was used to determine whether antibodies could facilitate infection and replication of the arenaviruses, Pichinde virus (PV) and Lassa fever virus (LFV). When high dilutions of PV-immune serum were added to cultures simultaneously with PV inoculum, virus replication was dramatically (1000-fold) increased. Low dilutions of this antiserum neutralized the virus. LFV also replicated in U937 cells. The presence of LFV-specific immune serum in the growth medium increased the viral titre as much as 10,000-fold. Addition of heat-aggregated IgG partially inhibited antibody-mediated enhancement, probably by inhibiting the binding of immune complexes to the monocytic cells.
U937单核细胞系被用于确定抗体是否能够促进沙粒病毒、皮钦德病毒（PV）和拉沙热病毒（LFV）的感染与复制。当将高稀释度的PV免疫血清与PV接种物同时加入培养物中时，病毒复制显著增加（1000倍）。该抗血清的低稀释度可中和病毒。LFV也能在U937细胞中复制。生长培养基中LFV特异性免疫血清的存在使病毒滴度增加多达10000倍。添加热聚集的IgG可部分抑制抗体介导的增强作用，这可能是通过抑制免疫复合物与单核细胞的结合来实现的。
