恒河猴衰老相关的基因表达和 DNA 甲基化变化

Age-related gene expression and DNA methylation changes in rhesus macaque.

机构信息

Key Laboratory of Bioresources and Ecoenvironment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, People's Republic of China.

Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, Sichuan, People's Republic of China.

出版信息

Genomics. 2020 Nov;112(6):5147-5156. doi: 10.1016/j.ygeno.2020.09.021. Epub 2020 Sep 11.

DOI:10.1016/j.ygeno.2020.09.021

PMID:32927008

Abstract

Aging is a very complicated biological process that can change gene expressions. The Chinese rhesus macaque (Macaca mulatta lasiota; CR) is closely related to humans. We explored gene expression with increasing age and DNA methylation changes in young and old CRs. Results showed blood transcriptome and DNA methylome significantly changed from young to old CRs. The age-associated differentially expressed genes (DEGs) and differentially methylated regions (DMRs) were associated with age-related biological features, such as immunity, blood coagulation, and biosynthetic process. The measurements of coagulation indicators confirmed old CRs had shorter coagulation time than young CRs, and the activities of coagulation factor II (FII) and factor VIII (FVIII) were enhanced in old CRs. Humans and CRs exhibited the same enhanced blood coagulation with age phenotype. Our study found aging is a critical factor affecting gene expression in CRs, and also provided new insights into the blood coagulation changes in non-human primates.

摘要

衰老是一个非常复杂的生物学过程，可以改变基因表达。中国猕猴（Macaca mulatta lasiota；CR）与人类密切相关。我们探索了年轻和年老 CR 随年龄增长的基因表达和 DNA 甲基化变化。结果表明，血液转录组和 DNA 甲基化组从年轻的 CR 到年老的 CR 发生了显著变化。与年龄相关的差异表达基因（DEGs）和差异甲基化区域（DMRs）与免疫、血液凝固和生物合成过程等与年龄相关的生物学特征相关。凝血指标的测量证实，年老的 CR 的凝血时间比年轻的 CR 短，并且年老的 CR 中凝血因子 II（FII）和因子 VIII（FVIII）的活性增强。人和 CR 随着年龄的增长表现出相同的增强血液凝固表型。我们的研究发现，衰老是影响 CR 基因表达的一个关键因素，也为非人类灵长类动物的血液凝固变化提供了新的见解。