凝乳酶原的完整氨基酸序列。
The complete amino acid sequence of prochymosin.
作者信息
Foltmann B, Pedersen V B, Jacobsen H, Kauffman D, Wybrandt G
出版信息
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2321-4. doi: 10.1073/pnas.74.6.2321.
Abstract
The total sequence of 365 amino acid residues in bovine prochymosin is presented. Alignment with the amino acid sequence of porcine pepsinogen shows that 204 amino acid residues are common to the two zymogens. Further comparison and alignment with the amino acid sequence of penicillopepsin shows that 66 residues are located at identical positions in all three proteases. The three enzymes belong to a large group of proteases with two aspartate residues in the active center. This group forms a family derived from one common ancestor.
摘要
本文给出了牛凝乳酶原365个氨基酸残基的完整序列。与猪胃蛋白酶原的氨基酸序列比对显示，这两种酶原共有204个氨基酸残基。进一步与青霉蛋白酶的氨基酸序列进行比较和比对表明，在所有这三种蛋白酶中，有66个残基位于相同位置。这三种酶属于一大类在活性中心含有两个天冬氨酸残基的蛋白酶。该类蛋白酶构成了一个源于共同祖先的家族。
相似文献
1
The complete amino acid sequence of prochymosin.凝乳酶原的完整氨基酸序列。
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2321-4. doi: 10.1073/pnas.74.6.2321.
2
The primary structure of calf chymosin.
J Biol Chem. 1979 Sep 10;254(17):8447-56.
3
Amino-acid sequence of the peptide segment liberated during activation of prochymosin (prorennin).凝乳酶原（前肾素）激活过程中释放的肽段的氨基酸序列。
Eur J Biochem. 1975 Jun 16;55(1):95-103. doi: 10.1111/j.1432-1033.1975.tb02141.x.
4
A basic residue at position 36p of the propeptide is not essential for the correct folding and subsequent autocatalytic activation of prochymosin.前凝乳酶原肽链第36位的碱性残基对于前凝乳酶的正确折叠及随后的自催化激活并非必需。
Eur J Biochem. 2001 Apr;268(8):2362-8. doi: 10.1046/j.1432-1327.2001.02119.x.
5
The primary structure and enzymic properties of porcine prochymosin and chymosin.
Int J Biochem Cell Biol. 1996 Jun;28(6):667-75. doi: 10.1016/1357-2725(96)00002-7.
6
Comparison between prochymosin and pepsinogen from lamb and calf.
Comp Biochem Physiol B. 1988;89(2):385-91. doi: 10.1016/0305-0491(88)90241-6.
7
Investigations on the activation of bovine prochymosin.牛凝乳酶原激活的研究。
Eur J Biochem. 1979 Mar;94(2):573-80. doi: 10.1111/j.1432-1033.1979.tb12927.x.
8
Prochymosin activation by non-aspartic proteinases.非天冬氨酸蛋白酶对凝乳酶原的激活作用。
FEBS Lett. 1990 Jan 29;260(2):173-5. doi: 10.1016/0014-5793(90)80096-2.
9
The first cleavage site in pepsinogen activation.
Adv Exp Med Biol. 1977;95:103-27. doi: 10.1007/978-1-4757-0719-9_7.
10
Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin.编码小牛前凝乳酶原的cDNA的分子克隆及核苷酸序列
Nucleic Acids Res. 1982 Apr 10;10(7):2177-87. doi: 10.1093/nar/10.7.2177.
引用本文的文献
1
Codon Optimization of the "Bos Taurus Chymosin" Gene for the Production of Recombinant Chymosin in Pichia pastoris.用于在毕赤酵母中生产重组凝乳酶的“牛凝乳酶”基因的密码子优化
Mol Biotechnol. 2016 Oct;58(10):657-664. doi: 10.1007/s12033-016-9965-7.
2
Mechanism of activation of the gastric aspartic proteinases: pepsinogen, progastricsin and prochymosin.胃天冬氨酸蛋白酶的激活机制：胃蛋白酶原、胃泌酸蛋白酶原和凝乳酶原。
Biochem J. 1998 Nov 1;335 ( Pt 3)(Pt 3):481-90. doi: 10.1042/bj3350481.
3
alpha-Bromo-4-amino-3-nitroacetophenone, a new reagent for protein modification. Modification of the methionine-290 residue of porcine pepsin.α-溴-4-氨基-3-硝基苯乙酮，一种用于蛋白质修饰的新试剂。猪胃蛋白酶甲硫氨酸-290残基的修饰
Biochem J. 1980 May 1;187(2):345-52. doi: 10.1042/bj1870345.
4
Amino acid sequence of porcine spleen cathepsin D.猪脾脏组织蛋白酶D的氨基酸序列。
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3703-7. doi: 10.1073/pnas.81.12.3703.
5
Synthesis of peptides related to the prosegment of mouse submaxillary gland renin precursor: an approach to renin inhibitors.与小鼠下颌下腺肾素前体前肽相关的肽的合成：一种肾素抑制剂的研究方法。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):48-52. doi: 10.1073/pnas.81.1.48.
6
Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis.解淀粉芽孢杆菌枯草杆菌蛋白酶在枯草芽孢杆菌中的克隆、测序及分泌
Nucleic Acids Res. 1983 Nov 25;11(22):7911-25. doi: 10.1093/nar/11.22.7911.
7
Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin.编码小牛前凝乳酶原的cDNA的分子克隆及核苷酸序列
Nucleic Acids Res. 1982 Apr 10;10(7):2177-87. doi: 10.1093/nar/10.7.2177.
8
Examination of calf prochymosin accumulation in Escherichia coli: disulphide linkages are a structural component of prochymosin-containing inclusion bodies.大肠杆菌中小牛凝乳酶原积累的检测：二硫键是含凝乳酶原的包涵体的结构成分。
EMBO J. 1985 Mar;4(3):775-80. doi: 10.1002/j.1460-2075.1985.tb03696.x.
9
Cloning and sequencing of a gene for Mucor rennin, an aspartate protease from Mucor pusillus.微小毛霉天冬氨酸蛋白酶——毛霉凝乳酶基因的克隆与测序
Nucleic Acids Res. 1986 Oct 10;14(19):7557-68. doi: 10.1093/nar/14.19.7557.
10
Characterization of bovine kappa-casein fractions and the kinetics of chymosin-induced macropeptide release from carbohydrate-free and carbohydrate-containing fractions determined by high-performance gel-permeation chromatography.通过高效凝胶渗透色谱法对牛κ-酪蛋白组分进行表征，并测定凝乳酶诱导的无碳水化合物和含碳水化合物组分中巨肽释放的动力学。
Biochem J. 1986 Nov 15;240(1):87-97. doi: 10.1042/bj2400087.
本文引用的文献
1
STUDIES ON RENNIN. 8. ON THE MOLECULAR WEIGHT OF PRORENNIN AND RENNIN.
C R Trav Lab Carlsberg. 1964;34:287-98.
2
3
Inhibition of cathepsin D by diazoacetylnorleucine methyl ester.重氮乙酰正亮氨酸甲酯对组织蛋白酶D的抑制作用。
FEBS Lett. 1970 Feb 25;6(4):312-314. doi: 10.1016/0014-5793(70)80086-2.
4
The sequence around an active-site aspartyl residue in pepsin.胃蛋白酶中活性位点天冬氨酰残基周围的序列。
FEBS Lett. 1968 Sep;1(4):211-212. doi: 10.1016/0014-5793(68)80063-8.
5
Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups.肽在纸上的电泳迁移率及其在酰胺基团测定中的应用。
Nature. 1966 Aug 6;211(5049):591-3. doi: 10.1038/211591a0.
6
Fragmentation of bovine chymotrypsinogen A and chymotrypsin A-alpha. Specific cleavage at arginine and methionine residues and separation of peptides, including B and C chains of chymotrypsin.
Biochemistry. 1970 Nov 10;9(23):4470-80. doi: 10.1021/bi00825a002.
7
The inactivation of pepsin by diazoacetylnorleucine methyl ester.重氮乙酰正亮氨酸甲酯对胃蛋白酶的失活作用。
J Biol Chem. 1966 Sep 25;241(18):4295-7.
8
A review on prorennin and rennin.关于前肾素和肾素的综述。
C R Trav Lab Carlsberg. 1966;35(8):143-231.
9
Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.用于埃德曼-贝格蛋白质测序仪的定量方法。两种异常免疫球蛋白轻链Rzf和Sac的部分序列。
Biochemistry. 1971 Dec 21;10(26):4912-21. doi: 10.1021/bi00802a013.
10
Strategy and tactics in protein chemistry.蛋白质化学中的策略与战术。
Biochem J. 1970 Oct;119(5):805-22. doi: 10.1042/bj1190805f.
Zotero 插件