Collings A, Davis B, Mills J
Department of Biological Sciences, Sheffield City Polytechnic, Great Britain.
Microbios. 1988;53(216-217):197-210.
Factors affecting the release of protoplasts from mycelia of Aspergillus fumigatus, Aspergillus niger, Penicillium ochrochloron and Geosmithia emersonii were investigated. Protoplast yields were found to be greatly dependent on the growth temperature, incubation conditions, lytic enzyme system, mycelial age and mycelial concentration. High yields of protoplasts were obtained from all four organisms after a 3 h incubation with 1 mg/ml Novozym 234 in 0.1 M 2-N-[morpholino] ethane sulphonic acid (MES) buffer at pH 5.0, containing 0.6 M sodium chloride. Supplementation of the incubation medium with 0.03 M calcium chloride enhanced protoplast release in P. ochrochloron but not in the other three fungi.
研究了影响烟曲霉、黑曲霉、淡绿青霉和艾默生土栖曲霉的菌丝体原生质体释放的因素。发现原生质体产量很大程度上取决于生长温度、培养条件、裂解酶系统、菌丝体年龄和菌丝体浓度。在pH 5.0的0.1 M 2-N-[吗啉代]乙烷磺酸（MES）缓冲液中，含有0.6 M氯化钠，用1 mg/ml诺维信234孵育3小时后，从所有四种生物体中都获得了高产的原生质体。在孵育培养基中添加0.03 M氯化钙可增强淡绿青霉中原生质体的释放，但对其他三种真菌无效。
